实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
21期
3418-3421
,共4页
陈江涛%林敏%刘配芬%钟德善%谢东德%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi%杨立业%杨辉%詹小芬%杨惠钿
陳江濤%林敏%劉配芬%鐘德善%謝東德%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi%楊立業%楊輝%詹小芬%楊惠鈿
진강도%림민%류배분%종덕선%사동덕%Santiago-m Monte-Nguba%Juan Carlos Salas Ehapo%Urbano Monsuy Eyi%양립업%양휘%첨소분%양혜전
疟疾, 恶性%G6PD缺乏症%横断面研究
瘧疾, 噁性%G6PD缺乏癥%橫斷麵研究
학질, 악성%G6PD결핍증%횡단면연구
Malaria,falciparum%Glucose-6-phosphate dehydrogenase deficiency%Cross-sectional study
目的：观察葡萄糖－6－磷酸脱氢酶（G6PD）缺陷症不同表型对恶性疟原虫感染的影响。方法：采用横断面研究的方式，将2012年雨季到马拉博地区医院就诊的2690名比奥克岛当地居民纳入研究。用显微镜镜检、荧光定量 PCR 结合熔解曲线法检测疟原虫。用荧光斑点法及 PCR-DNA 测序鉴定 G6PD 缺陷症。采用 Logistic 回归进行关联性分析。结果：该人群的 G6PD 缺陷症的总发生率为9．22％，基因型均为G6PD*A－（c．202 G＞A／c．376 A＞G）。G6PD缺陷症体现出强烈的疟疾保护作用（P＜0．05）；不同性别和表型的 G6PD 缺陷者中，只有男性半合子对疟疾有保护作用（P＜0．05），而女性杂合子组与女性纯合子组均无统计学差异（P＞0．05）。结论：G6PD 缺陷症的男性半合子表型能够减低恶性疟疾感染的风险，但是其机制尚未明确，有待更深入的研究。
目的：觀察葡萄糖－6－燐痠脫氫酶（G6PD）缺陷癥不同錶型對噁性瘧原蟲感染的影響。方法：採用橫斷麵研究的方式，將2012年雨季到馬拉博地區醫院就診的2690名比奧剋島噹地居民納入研究。用顯微鏡鏡檢、熒光定量 PCR 結閤鎔解麯線法檢測瘧原蟲。用熒光斑點法及 PCR-DNA 測序鑒定 G6PD 缺陷癥。採用 Logistic 迴歸進行關聯性分析。結果：該人群的 G6PD 缺陷癥的總髮生率為9．22％，基因型均為G6PD*A－（c．202 G＞A／c．376 A＞G）。G6PD缺陷癥體現齣彊烈的瘧疾保護作用（P＜0．05）；不同性彆和錶型的 G6PD 缺陷者中，隻有男性半閤子對瘧疾有保護作用（P＜0．05），而女性雜閤子組與女性純閤子組均無統計學差異（P＞0．05）。結論：G6PD 缺陷癥的男性半閤子錶型能夠減低噁性瘧疾感染的風險，但是其機製尚未明確，有待更深入的研究。
목적：관찰포도당－6－린산탈경매（G6PD）결함증불동표형대악성학원충감염적영향。방법：채용횡단면연구적방식，장2012년우계도마랍박지구의원취진적2690명비오극도당지거민납입연구。용현미경경검、형광정량 PCR 결합용해곡선법검측학원충。용형광반점법급 PCR-DNA 측서감정 G6PD 결함증。채용 Logistic 회귀진행관련성분석。결과：해인군적 G6PD 결함증적총발생솔위9．22％，기인형균위G6PD*A－（c．202 G＞A／c．376 A＞G）。G6PD결함증체현출강렬적학질보호작용（P＜0．05）；불동성별화표형적 G6PD 결함자중，지유남성반합자대학질유보호작용（P＜0．05），이녀성잡합자조여녀성순합자조균무통계학차이（P＞0．05）。결론：G6PD 결함증적남성반합자표형능구감저악성학질감염적풍험，단시기궤제상미명학，유대경심입적연구。
Objective To investigate the impact of Glucose-6-phosphate dehydrogenase (G6PD) deficiency on plasmodium falciparum malaria. Methods A cross-sectional study was performed on 2 690 patients in Malabo regional hospital on Bioko Island during rainy season (2012). The plasmodium falciparum was identified by real-time PCR and oil immersion microscopy. G6PD deficiency was identified by a fluorescent spot test (FST) and PCR-DNA sequencing. Logistic regression was conducted to estimate the association between G6PD deficiency and malaria. Results The prevalence of G6PD was 9.22% in the population , all of whose genotype G6PD deficiency was G6PD*A-(c.202 G > A/c.376 A > G). Confounding factors-adjusted OR showed that G6PD deficiency provided significant protection against malaria (P < 0.05). It was found that male hemizygotes has a protective effect against malaria (P < 0.05), while the female heterozygote group and female homozygous group had no statistical difference (P > 0.05). Conclusions The results suggest that male hemizygotes could provide protection against malaria. Further studies are required to explore the molecular mechanism in malaria infection.
