CAJ | 학술논문

目的：探讨大鼠骨髓间充质干细胞（MSCs）对LPS诱导BV2小胶质细胞炎症反应的影响。方法：采用贴壁分离法分离、纯化大鼠MSCs，BV2小胶质细胞进行常规培养传代。实验分为 PBS 对照组（A 组）、PBS 联合MSCs处理组（B组）、LPS 刺激组（C组）、LPS 联合MSCs干预组（D组）。 BV2小胶质细胞接种于6孔板中培养过夜后，按上述实验分组处理，24 h后收集各组培养上清液用于后续实验检测。显微镜下观察BV2小胶质细胞形态变化，Griess法检测NO浓度，ELISA法检测TNF-α、IL-1β的含量。结果：MSCs可以改善活化小胶质细胞的细胞形态；C组中LPS诱导小胶质细胞活化后，培养上清NO、IL-1β、TNF-α的含量明显上升（P＜0．05）；D组中予MSCs处理后NO、IL-β、TNF-α释放显著减少（P＜0．05）。结论：MSCs明显降低活化小胶质细胞炎症因子的释放，其可能是通过抑制小胶质细胞炎症反应而发挥神经保护作用；本研究中MSCs通过非直接接触的方式作用于小胶质细胞，推测没有进入脑内的MSCs对活化小胶质细胞亦有抑制作用。
목적：탐토대서골수간충질간세포（MSCs）대LPS유도BV2소효질세포염증반응적영향。방법：채용첩벽분리법분리、순화대서MSCs，BV2소효질세포진행상규배양전대。실험분위 PBS 대조조（A 조）、PBS 연합MSCs처리조（B조）、LPS 자격조（C조）、LPS 연합MSCs간예조（D조）。 BV2소효질세포접충우6공판중배양과야후，안상술실험분조처리，24 h후수집각조배양상청액용우후속실험검측。현미경하관찰BV2소효질세포형태변화，Griess법검측NO농도，ELISA법검측TNF-α、IL-1β적함량。결과：MSCs가이개선활화소효질세포적세포형태；C조중LPS유도소효질세포활화후，배양상청NO、IL-1β、TNF-α적함량명현상승（P＜0．05）；D조중여MSCs처리후NO、IL-β、TNF-α석방현저감소（P＜0．05）。결론：MSCs명현강저활화소효질세포염증인자적석방，기가능시통과억제소효질세포염증반응이발휘신경보호작용；본연구중MSCs통과비직접접촉적방식작용우소효질세포，추측몰유진입뇌내적MSCs대활화소효질세포역유억제작용。
Objective To explore the effect of bone marrow mesenchymal stem cells (MSCs) on LPS-stimulated BV2 microglia in inflammatory reaction. Methods Mouse MSCs were isolated and purified by adherence screening. The routinely cultured BV2 microglia in vitro were divided into PBS control group (group A),PBS plus MSCs treatment group(group B),LPS stimulation group(group C) and LPS plus MSCs group(group D).MSCs and BV2 microglia were cultured in the transwell co-culture system for 24 hours. We observed BV2 microglia morphological changes under the microscope,detected the concentrations of NO by Griess reaction,and the level of IL-1β,TNF-αby ELISA. Results MSCs can improve the morphology of activated microglia. The concentrations of TNF-a, IL-1βand N0 in culture supernatants were increased significantly (P < 0.05) after microglia activation, however, at the present of MSCs,the concentration of these inflammatory factors declined dramaticly (P<0.05). Conclusions MSCs can significantly inhibit the activation of microglia. It may play a neuroprotective effect by reducing the inflammation of microglia. MSCs showing anti-inflammatory effects through non-direct contact with nicroglial, suggesting that MSCs outside the brain may also inhibit the activation of microglia.