CAJ | 학술논문

目的：建立裂褶菌子实体中多糖、蛋白质含量的测定方法。方法采用分光光度法测定多糖和总蛋白含量，检测波长分别为479nm和595nm。结果多糖浓度在0.012~0.040mg/mL范围内具有良好的线性关系，回归方程为Y=23.0753X-0.1733，r=0.9998，子实体多糖含量为7.26%；蛋白浓度在0.0078~0.0321mg/mL范围内具有良好的线性关系，回归方程为Y=20.0830X+0.1451，r=0.9997，子实体总蛋白含量为0.2631%。结论本研究建立的方法简便、快速、准确，重复性好，为裂褶菌子实体多糖和蛋白质含量的测定提供了依据。
목적：건립렬습균자실체중다당、단백질함량적측정방법。방법채용분광광도법측정다당화총단백함량，검측파장분별위479nm화595nm。결과다당농도재0.012~0.040mg/mL범위내구유량호적선성관계，회귀방정위Y=23.0753X-0.1733，r=0.9998，자실체다당함량위7.26%；단백농도재0.0078~0.0321mg/mL범위내구유량호적선성관계，회귀방정위Y=20.0830X+0.1451，r=0.9997，자실체총단백함량위0.2631%。결론본연구건립적방법간편、쾌속、준학，중복성호，위렬습균자실체다당화단백질함량적측정제공료의거。
Objective Set up Schizophyllumcommune sporophore polysaccharide and protein content determination method. Methods Use spectrophotometry to determine polysaccharide and protein content, detection wavelength is 479nm and 595nm. Results Polysaccharide concentration possesses good linear relationship between 0.012-0.010mg/mL, regression equation is Y=23.0753X-0.1733, r=0.9998, sporophore polysaccharide content is 7.26%; protein concentration possesses good linear relationship between 0.0078-0.0321mg/mL, regression equation is Y=20.0830X+0.1451, r=0.9997, sporophore total protein is 0.2631%. Conclusion The method set up by the research is simple, fast, accurate and has good repeatability which provides basis for Schizophyllumcommune sporophore polysaccharide and protein content determination.