CAJ | 학술논문

磷脂酰丝氨酸具有改善大脑的认知和记忆的功能，是医药和食品行业重要的原料．为进一步提高大肠杆菌(Escherichia coli)中磷脂酰丝氨酸含量，利用 Red 重组系统，以可积累磷脂酰丝氨酸的 E. coli K12(?sdaA ?sdaB?pgpB)为出发菌株，敲除 pgpA 和 tdcG 基因，切断与磷脂酰丝氨酸合成相关的 L-丝氨酸和磷脂酰甘油磷酸的分解代谢途径．摇瓶发酵后的检测结果表明，重组菌株E. coli K12(?sdaA?sdaB?pgpA?pgpB?tdcG)中磷脂酰丝氨酸占菌体总磷脂的比例为2.5%，较出发菌株(1%)提高了1.5倍．
린지선사안산구유개선대뇌적인지화기억적공능，시의약화식품행업중요적원료．위진일보제고대장간균(Escherichia coli)중린지선사안산함량，이용 Red 중조계통，이가적루린지선사안산적 E. coli K12(?sdaA ?sdaB?pgpB)위출발균주，고제 pgpA 화 tdcG 기인，절단여린지선사안산합성상관적 L-사안산화린지선감유린산적분해대사도경．요병발효후적검측결과표명，중조균주E. coli K12(?sdaA?sdaB?pgpA?pgpB?tdcG)중린지선사안산점균체총린지적비례위2.5%，교출발균주(1%)제고료1.5배．
Phosphatidylserine(PS)is an important active ingredient for medicine and food industry which could improve the cognitive ability and memory. In order to improve the content of PS in Escherichia coli, the Red recombinant system was used for deleting the gene pgpA and tdcG in E. coli K12(?sdaA ?sdaB ?pgpB)which can accumulate PS to block the L-serine degradation and cutting off the phosphatidylglycerophosphate catabolic pathway. After fermentation,the results showed that the total PS phospholipids synthesized by the mutant strain E. coli K12(?pgpA?pgpB?sdaA?sdaB?tdcG)was 2.5%which was 1.5 times higher than that in the starting strain(1%).