CAJ | 학술논문

人的SLP-2基因在乳腺癌组织的高表达与病人的存活率相关。为了研究SLP-2在乳腺癌中的功能，应用免疫印记的方法检测SLP-2在不同乳腺癌细胞中的蛋白表达水平，发现随着细胞癌化程度的增强其表达也增强。成功构建pCMV-Myc-SLP-2重组质粒，证明了其在MCF-7细胞的表达，MTT实验结果表明SiRNA转染乳腺癌细胞MCF-7可以抑制细胞增殖，同时发现SLP-2在乳腺癌细胞系中表达量与HER2／nue呈负相关。这些结果为深入研究乳腺癌的发生和发展奠定了基础。
인적SLP-2기인재유선암조직적고표체여병인적존활솔상관。위료연구SLP-2재유선암중적공능，응용면역인기적방법검측SLP-2재불동유선암세포중적단백표체수평，발현수착세포암화정도적증강기표체야증강。성공구건pCMV-Myc-SLP-2중조질립，증명료기재MCF-7세포적표체，MTT실험결과표명SiRNA전염유선암세포MCF-7가이억제세포증식，동시발현SLP-2재유선암세포계중표체량여HER2／nue정부상관。저사결과위심입연구유선암적발생화발전전정료기출。
High-level Human stomatin like protein 2 ( SLP-2 ) expression was associated with significantly de-creased survival in breast cancer.To study the function of SLP-2 in breast cancer furtherly, the expression of SLP-2 protein was detected in different breast cancer cells by western blotting, and the expression of its protein in breast cancer cells was found to increase with the increase of the degree of cancer.Recombinant plasmid pCMV-Myc-SLP-2 was successfully constructed and demonstrated that it can express well in MCF-7.The MTT assay showed that the cell proliferation was significantly inhibited after transfection of pCMV-Myc-SLP-2 , the expression of SLP-2 was negatively correlated with HER2/nue in MCF-7, thus providing a useful clue to further research on the occurring and development of breast cancer.