CAJ | 학술논문

目的：探讨CD64对白血病合并细菌感染的早期诊断价值。方法选取白血病合并细菌感染患者50例、白血病非细菌感染患者48例和体检正常者38例，静脉抽取外周血，分别用流式细胞术检测CD64的表达及CD4／CD8比值，免疫比浊法检测降钙素原（PCT）和C‐反应蛋白（CRP），以及电阻抗法检测中性粒细胞百分比（NEU％）。结果①白血病合并细菌感染组CD64的表达显著高于白血病非细菌感染组和正常对照组，差异具有统计学意义（ P＜0.01）。②白血病合并细菌感染组的淋巴细胞CD4／CD8值要显著低于白血病非细菌感染组和正常对照组，差异具有统计学意义（P＜0.01），提示其细胞免疫功能受到抑制。③从ROC曲线可以得出，PCT作为诊断早期感染的指标准确性优于CRP和NEU％，而CD64诊断早期合并细菌感染时的特异度和敏感度略高于PCT ，所以CD64在诊断早期合并细菌感染时显示了更大的优势。结论 CD64对于白血病合并细菌感染具有良好的特异度，其对白血病合并细菌感染的早期诊断具有重要价值。
목적：탐토CD64대백혈병합병세균감염적조기진단개치。방법선취백혈병합병세균감염환자50례、백혈병비세균감염환자48례화체검정상자38례，정맥추취외주혈，분별용류식세포술검측CD64적표체급CD4／CD8비치，면역비탁법검측강개소원（PCT）화C‐반응단백（CRP），이급전조항법검측중성립세포백분비（NEU％）。결과①백혈병합병세균감염조CD64적표체현저고우백혈병비세균감염조화정상대조조，차이구유통계학의의（ P＜0.01）。②백혈병합병세균감염조적림파세포CD4／CD8치요현저저우백혈병비세균감염조화정상대조조，차이구유통계학의의（P＜0.01），제시기세포면역공능수도억제。③종ROC곡선가이득출，PCT작위진단조기감염적지표준학성우우CRP화NEU％，이CD64진단조기합병세균감염시적특이도화민감도략고우PCT ，소이CD64재진단조기합병세균감염시현시료경대적우세。결론 CD64대우백혈병합병세균감염구유량호적특이도，기대백혈병합병세균감염적조기진단구유중요개치。
Objective To explore early diagnostic value of CD64 in leukemia complicated with bacterial infection.Methods Fifty leukemia patients complicated with bacterial infection ,48 leukemia patients without bacterial infection and 38 normal con‐trol group were enrolled into this study.Peripheral venous blood was collected.Expression of CD64 and the ratio of CD4/CD8 were detected by flow cytometry.Immunoturbidimetric assay was adopted to detect procalcitonin(PCT)and C‐reactive protein (CRP) ,and bioelectrlcal impedance analysis used to determine neutrophilic granulocyte percentage(NEU% ).Results ①CD64 expression was significantly higher in the leukemia patients complicated with bacterial infection than in the other two groups(P<0.01). ②The ratio of CD4 to CD8 was significantly lower in the leukemia patients complicated with bacterial infection group than in the other two groups(P<0.01). ③The ROC curve showed that accuracy of PCT as an early diagnosis indicator for early infection was superior to CRP and NEU% ,and the specificity and sensitivity of CD64 in early diagnosis of leukemia combined with bacterial infection were higher than those of PCT.So CD64 had an advantage of early diagnosis in leukemia combined with bacterial infection.Conclusion CD64 has high specificity in diagnosis of bacterial infection ,and it played an important role in early diagnosis of leukemia complicated with bacterial infection.