华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2014年
6期
648-652
,共5页
赵艳%金鑫%于东红%钱军
趙豔%金鑫%于東紅%錢軍
조염%금흠%우동홍%전군
胃肿瘤%乙醛脱氢酶1%幽门螺杆菌 L型%肿瘤干细胞
胃腫瘤%乙醛脫氫酶1%幽門螺桿菌 L型%腫瘤榦細胞
위종류%을철탈경매1%유문라간균 L형%종류간세포
stomach neoplasms%aldehyde dehydrogenase 1%Helicobacter pylori L-form%cancer stem cell
目的：探讨乙醛脱氢酶1（ALDH1）在人胃癌组织中的表达与幽门螺杆菌 L型（H p‐L型）感染的关系。方法应用免疫组化Elivision法和原位杂交方法检测126例胃癌及30例正常对照胃组织ALDH1的表达，应用革兰染色和免疫组化法检测上述组织的 H p‐L型感染情况。结果胃癌组织中ALDH1蛋白和mRNA的表达阳性率分别为53.2％和56.3％，与对照组之间差异具有统计学意义（均 P＜0.01），ALDH1蛋白的表达与肿瘤的临床 TNM 分期和淋巴结转移密切相关（均 P＜0.01）。胃癌及对照组中 Hp‐L 型的阳性率分别为67.5％和23.3％，差异具有统计学意义（P＜0.01）。胃癌组 H p‐L型感染阳性者的ALDH1蛋白表达阳性率高于 H p‐L 型感染阴性组（P＜0.05）。ALDH1蛋白的表达与 H p‐L型感染呈正相关（rs ＝0.220，P＜0.05）。ALDH1阳性患者5年生存率明显低于ALDH1阴性患者（P＜0.05）。结论 ALDH1在胃癌组织中高表达，可作为评价胃癌预后的指标，H p‐L型感染可促进其表达。
目的：探討乙醛脫氫酶1（ALDH1）在人胃癌組織中的錶達與幽門螺桿菌 L型（H p‐L型）感染的關繫。方法應用免疫組化Elivision法和原位雜交方法檢測126例胃癌及30例正常對照胃組織ALDH1的錶達，應用革蘭染色和免疫組化法檢測上述組織的 H p‐L型感染情況。結果胃癌組織中ALDH1蛋白和mRNA的錶達暘性率分彆為53.2％和56.3％，與對照組之間差異具有統計學意義（均 P＜0.01），ALDH1蛋白的錶達與腫瘤的臨床 TNM 分期和淋巴結轉移密切相關（均 P＜0.01）。胃癌及對照組中 Hp‐L 型的暘性率分彆為67.5％和23.3％，差異具有統計學意義（P＜0.01）。胃癌組 H p‐L型感染暘性者的ALDH1蛋白錶達暘性率高于 H p‐L 型感染陰性組（P＜0.05）。ALDH1蛋白的錶達與 H p‐L型感染呈正相關（rs ＝0.220，P＜0.05）。ALDH1暘性患者5年生存率明顯低于ALDH1陰性患者（P＜0.05）。結論 ALDH1在胃癌組織中高錶達，可作為評價胃癌預後的指標，H p‐L型感染可促進其錶達。
목적：탐토을철탈경매1（ALDH1）재인위암조직중적표체여유문라간균 L형（H p‐L형）감염적관계。방법응용면역조화Elivision법화원위잡교방법검측126례위암급30례정상대조위조직ALDH1적표체，응용혁란염색화면역조화법검측상술조직적 H p‐L형감염정황。결과위암조직중ALDH1단백화mRNA적표체양성솔분별위53.2％화56.3％，여대조조지간차이구유통계학의의（균 P＜0.01），ALDH1단백적표체여종류적림상 TNM 분기화림파결전이밀절상관（균 P＜0.01）。위암급대조조중 Hp‐L 형적양성솔분별위67.5％화23.3％，차이구유통계학의의（P＜0.01）。위암조 H p‐L형감염양성자적ALDH1단백표체양성솔고우 H p‐L 형감염음성조（P＜0.05）。ALDH1단백적표체여 H p‐L형감염정정상관（rs ＝0.220，P＜0.05）。ALDH1양성환자5년생존솔명현저우ALDH1음성환자（P＜0.05）。결론 ALDH1재위암조직중고표체，가작위평개위암예후적지표，H p‐L형감염가촉진기표체。
Objective To investigate the relationship between ALDH1 expression and Helicobacter pylori L(Hp‐L)‐form infection in gastric carcinoma.Methods In 126 cases with gastric carcinoma and 30 healthy individuals ,expression of ALDH1 protein and mRNA was examined by immunohistochemical staining with Elivision method and in situ hybridization ,respective‐ly.In addition to the above tissues ,Hp‐Lwas detected by means of Gram stain and immunohistochemical staining with Elivision method.Results It was found that in gastric carcinoma ,the positive rate of ALDH1 protein and mRNA was 53.2% and 56.3% ,respectively ,significantly higher than that of normal controls(P< 0.01).The expression of protein was significantly correlated with TNM stage and lymph node metastasis(P<0.01).In these gastric carcinoma cases ,the positive rate for Hp‐L (67.5 % )was higher than that in the normal controls(23.3% )(P<0.01).ALDH1 protein expression was higher in the Hp‐L positive cases than in the H p‐L negative cases(P<0.05). There was positive correlation between ALDH1 expression and posi‐tive Hp‐L staining(rs =0.220 ,P<0.05).Kaplan‐Meier survival analysis showed that the patients with positive expression of ALDH1 had lower 5‐year overall survival rate than those with negative expression(P<0.05).Conclusion There is higher ex‐pression of ALDH1 in gastric carcinoma. It could serve as an important factor for the prognosis of gastric carcino‐ma. Helicobacter pylori L‐form may be associated with the up‐regulation of ALDH1.
