华中科技大学学报（医学版）
華中科技大學學報（醫學版）
화중과기대학학보（의학판）
ACTA UNIVERSITATIS MEDICINAE TONGJI
2014年
6期
639-642
,共4页
降钙素基因相关肽%奥沙利铂%外周神经病变
降鈣素基因相關肽%奧沙利鉑%外週神經病變
강개소기인상관태%오사리박%외주신경병변
calcitonin gene-related peptide%Oxaliplatin%peripheral neuropathy
目的：观察奥沙利铂诱发外周神经病变时大鼠的疼痛行为学改变以及降钙素基因相关肽（calcitonin gene‐re‐lated peptide ，CGRP）的表达情况。方法20只Wistar大鼠随机分成对照组和实验组，实验组予以腹腔注射奥沙利铂（20 mg／kg）造模。分别在造模前、造模后6、24、72 h和7 d ，检测大鼠机械性痛阈和冷痛阈，应用Western blot及免疫组化检测脊髓背根神经节中CGRP蛋白的表达。结果造模后6 h大鼠机械性痛阈较正常对照组降低[（11.6±2.2）g vs.（13.9±3.7）g]，并随着观察时间的延长而下降，在72 h降至低点（3.9±1.4）g ，一直持续到7 d ，冷刺激缩足潜伏期也呈现同样趋势的变化。奥沙利铂化疗后，大鼠脊髓背根神经节中CGRP表达明显升高，蛋白表达上调，并且与机械痛阈呈正相关。结论 CGRP在奥沙利铂诱导的外周神经病变大鼠中枢敏化中发挥重要促进作用。
目的：觀察奧沙利鉑誘髮外週神經病變時大鼠的疼痛行為學改變以及降鈣素基因相關肽（calcitonin gene‐re‐lated peptide ，CGRP）的錶達情況。方法20隻Wistar大鼠隨機分成對照組和實驗組，實驗組予以腹腔註射奧沙利鉑（20 mg／kg）造模。分彆在造模前、造模後6、24、72 h和7 d ，檢測大鼠機械性痛閾和冷痛閾，應用Western blot及免疫組化檢測脊髓揹根神經節中CGRP蛋白的錶達。結果造模後6 h大鼠機械性痛閾較正常對照組降低[（11.6±2.2）g vs.（13.9±3.7）g]，併隨著觀察時間的延長而下降，在72 h降至低點（3.9±1.4）g ，一直持續到7 d ，冷刺激縮足潛伏期也呈現同樣趨勢的變化。奧沙利鉑化療後，大鼠脊髓揹根神經節中CGRP錶達明顯升高，蛋白錶達上調，併且與機械痛閾呈正相關。結論 CGRP在奧沙利鉑誘導的外週神經病變大鼠中樞敏化中髮揮重要促進作用。
목적：관찰오사리박유발외주신경병변시대서적동통행위학개변이급강개소기인상관태（calcitonin gene‐re‐lated peptide ，CGRP）적표체정황。방법20지Wistar대서수궤분성대조조화실험조，실험조여이복강주사오사리박（20 mg／kg）조모。분별재조모전、조모후6、24、72 h화7 d ，검측대서궤계성통역화랭통역，응용Western blot급면역조화검측척수배근신경절중CGRP단백적표체。결과조모후6 h대서궤계성통역교정상대조조강저[（11.6±2.2）g vs.（13.9±3.7）g]，병수착관찰시간적연장이하강，재72 h강지저점（3.9±1.4）g ，일직지속도7 d ，랭자격축족잠복기야정현동양추세적변화。오사리박화료후，대서척수배근신경절중CGRP표체명현승고，단백표체상조，병차여궤계통역정정상관。결론 CGRP재오사리박유도적외주신경병변대서중추민화중발휘중요촉진작용。
Objective To observe the pain behavior of rats with Oxaliplatin‐induced peripheral neuropathy(OXIPN)and cal‐citonin gene related peptide(CGRP)expression.Methods Twenty Wistar rats were randomly divided into 2 groups :normal con‐trol group and model group.Oxaliplatin(20 mg/kg)was injected intraperitoneally to the rats in the model group.Mechanical pain threshold and cold allodynia were detected before and 6 ,24 ,72 h and 7 days after modeling ,respectively.Western blot and im‐munohistochemical method were employed to measure the expression of CGRP in dorsal spinal root ganglion of control group and model group.Results The mechanical pain threshold of modal group rats decreased 6 h after injection of Oxaliplatin as compared with the normal group[(11.6 ± 2.2)g vs. (13.9 ± 3.7)g] ,and decreased with prolongation of time ,reduced to the lowest at 72 h[(3.9 ± 1.4)g]and maintained to day 7 ,and the same trend was found in cold chemical allodynia test.CGRP ex‐pression of dorsal root ganglion increased significantly in the model group rats ,and there was a significant positive correlation between the expression of CGRP and the degree of mechanical pain threshold.Conclusion CGRP plays an important role in Ox‐aliplatin‐induced central sensitization of rats with peripheral neuropathy.
