CAJ | 학술논문

目的：建立生物检材血液中农药的快速溶剂萃取（accelerated solvent extraction，ASE）法。方法通过优化ASE萃取条件，考察萃取温度、时间和萃取剂对回收率的影响，提取血液中的农药进行气相色谱-质谱联用（GC/MS）法定性定量分析。结果血液中8种农药的平均回收率在70.6%~92.4%，变异系数小于5.0%，8种农药在0.5~5.0μg/mL的浓度范围内线性良好。结论该方法具有操作简便快捷、回收率高、重现性好等特点，可用于农药的提取检验。
목적：건립생물검재혈액중농약적쾌속용제췌취（accelerated solvent extraction，ASE）법。방법통과우화ASE췌취조건，고찰췌취온도、시간화췌취제대회수솔적영향，제취혈액중적농약진행기상색보-질보련용（GC/MS）법정성정량분석。결과혈액중8충농약적평균회수솔재70.6%~92.4%，변이계수소우5.0%，8충농약재0.5~5.0μg/mL적농도범위내선성량호。결론해방법구유조작간편쾌첩、회수솔고、중현성호등특점，가용우농약적제취검험。
Objective To develop the accelerated solvent extraction (ASE ) for determ ining pesticides pre-sent in blood sam ples. Methods Pesticides were extracted by ASE with optimized param eters to study recovery rate affected by extraction tem perature, time and agent. GC/MS was used to perform quantita-tive analysis.Results The recovery rates of eight pesticides were 70.6%-92.4%. The coefficient of variation was less than 5.0%. Agood linear relationship was obtained at the concentration range of 0.5-5.0μg/m L . Conclusion The m ethod was fast and sim ple with high recovery rate and good repeatability. It can be applied to analyze pesticides present in the blood specimen.