CAJ | 학술논문

目的：探讨大鼠皮肤切创纤维连接蛋白EIIIA片段的表达与损伤时间的关系。方法取48只成年SD大鼠并切割大鼠背部皮肤致伤。于伤后即刻、0.5h、1h、2h、3h、4h、6h、8h共8个时间段分别处死大鼠，取其创伤周围皮肤，利用免疫组织化学方法和Western印迹法检测EIIIA+纤维连接蛋白，观察其表达水平与损伤时间的关系。结果伤后即刻未出现EIIIA+纤维连接蛋白的表达，伤后0.5 h，皮肤基底细胞开始出现阳性表达，随损伤时间的延长，阳性着色持续增强。 Western印迹法检测发现，所测得的相对光密度值随损伤时间的延长而逐渐增高。结论 EIIIA片段表达变化可以作为法医学早期损伤时间的推断指标。
목적：탐토대서피부절창섬유련접단백EIIIA편단적표체여손상시간적관계。방법취48지성년SD대서병절할대서배부피부치상。우상후즉각、0.5h、1h、2h、3h、4h、6h、8h공8개시간단분별처사대서，취기창상주위피부，이용면역조직화학방법화Western인적법검측EIIIA+섬유련접단백，관찰기표체수평여손상시간적관계。결과상후즉각미출현EIIIA+섬유련접단백적표체，상후0.5 h，피부기저세포개시출현양성표체，수손상시간적연장，양성착색지속증강。 Western인적법검측발현，소측득적상대광밀도치수손상시간적연장이축점증고。결론 EIIIA편단표체변화가이작위법의학조기손상시간적추단지표。
Objective To explore the relationship between the expression of EIIIA + fibronectin in incised wound of rat’s skin and injury time. Methods The wounding model was established by cutting the dor-salskin of 48 adult SD rats. The rats were sacrificed atthe pre-setinjury time as immediately, 0. 5h, 1h, 2 h, 3 h, 4 h, 6 h, and 8 h. The skin sam ples were taken at the m argin of wound. The expression of the EIIIA + fibronectin was detected by im m unohistochem istry and W estern blotting and the relationship be-tween its expression and injury time was observed. Results The expression of EIIIA + fibronectin was not observed im m ediately. The basal cell of skin began to showpositive expression 0. 5 h after injury. W ith the extension of injury time, positive staining became stronger. The value of relative optical density was gradually increased with prolonged injury time by the W estern blotting analysis. Conclusion The expres-sion of EIIIA + fibronectin could be used for estimation of injury time in the early stage of skin injury.