CAJ | 학술논문

本研究以30个甘蔗栽培种或原种为材料，用 ISSR 标记方法进行了遗传多态性分析。结果表明：供试30份甘蔗种质材料的遗传相似性系数在0.375～1.000之间，其中，甘蔗栽培种与热带种的遗传相似性系数在0.850～0.950之间，说明这些甘蔗栽培种与热带种的血缘关系近；甘蔗栽培种与割手密之间遗传相似性系数在0.525～0.675之间，说明这些甘蔗栽培种与割手密有一定的血缘关系；甘蔗栽培种与斑茅的遗传相似性系数为在0.375～0.525之间，表明这些甘蔗栽培品种与斑茅的血缘关系较远(实质上参试的甘蔗品种都不是斑茅的后代)；参试的23份栽培种之间的遗传相似性系数关系在0.725～1.000之间，说明这些甘蔗栽培种之间的血缘关系较近，种质血缘窄，应加强含有多个野生种质的杂交后代的选育。同时，聚类分析结果表明，在遗传相似性系数为0.720水平上可将30份供试甘蔗种质材料分为3个类群，其中3个热带种和23个栽培种聚为一类，3个割手密聚为一类，斑茅单独聚为一类。
본연구이30개감자재배충혹원충위재료，용 ISSR 표기방법진행료유전다태성분석。결과표명：공시30빈감자충질재료적유전상사성계수재0.375～1.000지간，기중，감자재배충여열대충적유전상사성계수재0.850～0.950지간，설명저사감자재배충여열대충적혈연관계근；감자재배충여할수밀지간유전상사성계수재0.525～0.675지간，설명저사감자재배충여할수밀유일정적혈연관계；감자재배충여반모적유전상사성계수위재0.375～0.525지간，표명저사감자재배품충여반모적혈연관계교원(실질상삼시적감자품충도불시반모적후대)；삼시적23빈재배충지간적유전상사성계수관계재0.725～1.000지간，설명저사감자재배충지간적혈연관계교근，충질혈연착，응가강함유다개야생충질적잡교후대적선육。동시，취류분석결과표명，재유전상사성계수위0.720수평상가장30빈공시감자충질재료분위3개류군，기중3개열대충화23개재배충취위일류，3개할수밀취위일류，반모단독취위일류。
ISSR molecular markers were applied to analyze the genetic polymorphism of 30 sugarcane clones. The results showed that the genetic similarity coefficient among the selected sugarcane germplasm materials were between 0.375~1.000. Those between sugarcane cultivars and Saccharum officinarum were 0.850~0.950, indicating close consanguinity. Those between sugarcane cultivars and Saccharum spontaneum L. were 0.525~0.675, indicating some consanguinity. Those between sugarcane cultivars and Saccharum arundinaceum were 0.375~0.525, indicating remote consanguinity (as a matter of fact, all the cultivars used in the experiment is not the descendants of Saccharum arundinaceum). The genetic similarity coefficient among the 23 cultivars were 0.725~1.000, indicating close kinship and narrow genetic diversity, and therefore multiple wild germplasm should be introduced into cane hybrids in the future breeding. The cluster analysis on the level of genetic similarity coefficient of 0.720 classified the 30 sugarcane germplasm materials into 3 groups: Saccharum officinarum and cultivars, Saccharum spontaneum L., and Saccharum arundinaceum.