CAJ | 학술논문

研究人参皂苷在正丁醇／水二相中的分配规律，为人参皂苷的提取分离和分析提供依据。人参皂苷提取液经水饱和正丁醇萃取后，用HPLC／MS法检测中性人参皂苷Rb1、Rb2、Rc、Rd、Rg1、Re、Rf、Ro以及9种丙二酰基人参皂苷在正丁醇／水二相中的含量。不同人参皂苷在正丁醇和水二相中的分配比例有很大的差异，其中，丙二酰基人参皂苷在水中的分配比例大于在正丁醇中的分配比例，中性人参皂苷在水中的分配比例小于在正丁醇中的分配比例。经过3次水饱和正丁醇萃取，中性人参皂苷基本上全部转移到正丁醇相，丙二酰基人参皂苷转移到正丁醇相和水相中的含量之比为3∶2。
연구인삼조감재정정순／수이상중적분배규률，위인삼조감적제취분리화분석제공의거。인삼조감제취액경수포화정정순췌취후，용HPLC／MS법검측중성인삼조감Rb1、Rb2、Rc、Rd、Rg1、Re、Rf、Ro이급9충병이선기인삼조감재정정순／수이상중적함량。불동인삼조감재정정순화수이상중적분배비례유흔대적차이，기중，병이선기인삼조감재수중적분배비례대우재정정순중적분배비례，중성인삼조감재수중적분배비례소우재정정순중적분배비례。경과3차수포화정정순췌취，중성인삼조감기본상전부전이도정정순상，병이선기인삼조감전이도정정순상화수상중적함량지비위3∶2。
Ginsenosides distribution rules in n -butanol phase and water phase were studied ,to provide the basis for separation and analysis of ginsenosides .After solution of ginseng root was extracted with n -butanol saturated water ,ginsenoside content Rb1 ,Rb2 ,Rc ,Rd ,Rg1 ,Re ,Rf , Ro in two phases were detected with HPLC/MS ,as well as 9 kinds of malonyl-ginsenosides .Results:Distribution ratio of different ginsenosides in the biphase vary greatly ,malonyl-ginsenosides distribution ratio in water phase was larger than that in n -butanol phase while neutral gin-senosides appears a different side .Conclusion:After extracted three times with n -butanol saturated water ,substantially all of the neutral gin-senosides were transferred to n -butanol phase;the ratio of malonyl -ginsenosides in n-butanol phase to water phase is 3∶2 .