CAJ | 학술논문

研究了溶剂、波长、pH 对调味品中桔霉素吸收光谱的影响,并报道了采用荧光分光光度法对调味品中桔霉素含量进行测定的新方法．采用60％乙醇为溶剂对样品进行抽提,旋转蒸发浓缩,在激发波长300 nm、发射波长501 nm下测定调味品中桔霉素含量．根据标准曲线方程Y＝20.308X＋7.6026,线性相关系数R2＝0.9922,检出限为0.03 ug/mL,精密度(RSD)为6.81％,加标回收率为81.26％~90.40％．该法快速简便、成本低、灵敏度高,适合用于调味品中桔霉素含量的测定．
연구료용제、파장、pH 대조미품중길매소흡수광보적영향,병보도료채용형광분광광도법대조미품중길매소함량진행측정적신방법．채용60％을순위용제대양품진행추제,선전증발농축,재격발파장300 nm、발사파장501 nm하측정조미품중길매소함량．근거표준곡선방정Y＝20.308X＋7.6026,선성상관계수R2＝0.9922,검출한위0.03 ug/mL,정밀도(RSD)위6.81％,가표회수솔위81.26％~90.40％．해법쾌속간편、성본저、령민도고,괄합용우조미품중길매소함량적측정．
Comprehensive method for determining the content of citrinin in condiment was reported.The influence of the pH,solvent and wavelength on the stability of citrinin was investigated.The samples were extracted with 60% ethanol and enriched by rotary evaporator.The excitation and emission wave-lengths in the measurement were 300 nm,501 nm respectively.In this interval,the calibration curve isY=20.308X+7.602 6,andR2 is 0.992 2,RSD was 6.81%,the limit of detection is 0.03 ug/mg and the av-erage recovery is 81.26%~90.4%.This method was simple and accurate,which can be used for detec-ting citrinin in condiment.