CAJ | 학술논문

从大曲中分离筛选获得1株产3-羟基丁酮的菌株 G7,以葡萄糖为主要碳源,此菌株在38℃,180 r/min恒温振荡培养96 h,3-羟基丁酮产量达到24.30 g/L．根据形态特征、生理生化特征及16S rDNA序列分析,该菌株被鉴定为甲基营养型芽胞杆菌(Bacillus methylotrophicus strain )．将该菌株作为出发菌株,采用常压室温等离子体(Atmospheric and Room Temperature Plasma,简称 ARTP)诱变,并进行48孔板初筛及摇瓶复筛,同时对所获菌株进行遗传稳定性分析,最后得到1株遗传性状稳定的3-羟基丁酮高产菌株 G7-6,其产量可达31.65 g/L,比诱变前提高了30.25％,且转化率较高,为27.31％．
종대곡중분리사선획득1주산3-간기정동적균주 G7,이포도당위주요탄원,차균주재38℃,180 r/min항온진탕배양96 h,3-간기정동산량체도24.30 g/L．근거형태특정、생리생화특정급16S rDNA서렬분석,해균주피감정위갑기영양형아포간균(Bacillus methylotrophicus strain )．장해균주작위출발균주,채용상압실온등리자체(Atmospheric and Room Temperature Plasma,간칭 ARTP)유변,병진행48공판초사급요병복사,동시대소획균주진행유전은정성분석,최후득도1주유전성상은정적3-간기정동고산균주 G7-6,기산량가체31.65 g/L,비유변전제고료30.25％,차전화솔교고,위27.31％．
An acetoin-producing bacterial strain G7 was isolated from Daqu.When it was incubated for 96 h at 38 ℃ with shaking by 180 r/min,acetoin concentration of strain G7 could reach 24.30 g/L using glucose as the carbon source.Based on morphology,physiological and biochemical characteristics as well as the 16S rDNA gene sequence,strain G7 was identified as Bacillus methylotrophicus strain.As the o-riginal strain,G7 was mutagenized by ARTP.The mutant was preliminarily screened in 48-well micro-plates and further screened in shake flask,and evaluated for genetic stability.A high acetoin-producing strain named G7-6 with stable genetic characters was obtained.The acetoin product ion was 3 1.6 5 g/L, which was 30.25% higher than the original strain G7,and the conversion of acetoin can reach 27.31%.