CAJ | 학술논문

本文采用胶体几丁质为底物，研究了红曲霉液态发酵产几丁质酶．研究结果表明：胶体几丁质的添加能有效诱导红曲霉合成几丁质酶．在培养基中添加0.8％(W/V)胶体几丁质的同时，添加0.4％(W/V)葡萄糖，有利于红曲霉的生长以及几丁质酶的合成，发酵48 h几丁质酶活力达到最高的0.3504U/mL．
본문채용효체궤정질위저물，연구료홍곡매액태발효산궤정질매．연구결과표명：효체궤정질적첨가능유효유도홍곡매합성궤정질매．재배양기중첨가0.8％(W/V)효체궤정질적동시，첨가0.4％(W/V)포도당，유리우홍곡매적생장이급궤정질매적합성，발효48 h궤정질매활력체도최고적0.3504U/mL．
The study aimed to explore the chitinase production by Monascus liquid ferment-ation.The chitinase enzyme assay was carried out with colloidal chitin as a substrate.The experimental results indi-cate that addition of colloidal chitin helps the accumulation of chitinase.The highest activity of chitinase of 0.350 4 U/mL was obtained at 48 hours in themedium with the glucose 0.4%(W/V)and colloidal chitin 0.8%(W/V).