医学与法学
醫學與法學
의학여법학
Medicine and Jurisprudence
2014年
6期
77-78
,共2页
人脾破裂损伤%法医病理学%时间推断
人脾破裂損傷%法醫病理學%時間推斷
인비파렬손상%법의병이학%시간추단
human spleen rupture%forensic pathology%time estimation
为观察和分析法医病理学推断人脾破裂损伤时间的方法和特点。本文选取2010年6月至2013年10月120例外伤性脾破裂手术切除标本进行实验研究；按照标本破裂时间，把120例标本分成8组，采用常规染色体观察标本破裂出血区域边缘组织学的变化情况，经免疫组织染色后观察标本破裂出血区域SMA阳性细胞的表达规律。结果显示，损伤时间约6h组的标本有纤维素渗出，7~12h组有大量中性粒细胞浸润，13h~1d组分散少量SMA阳性细胞，1~2d组含有的SMA阳性细胞呈网状分布，2~3d组含有大量SMA阳性细胞，3~7d组和8~14d组出现肉芽组织，跃14d组的标本有瘢痕形成，各组标本SMA阳性细胞的数量差异具有统计学意义(P<0.05)。以脾脏标本破裂出血区域边缘组织学的变化情况，推断人脾破裂损伤时间的法医病理学鉴定方法具有一定的准确性和可靠性。
為觀察和分析法醫病理學推斷人脾破裂損傷時間的方法和特點。本文選取2010年6月至2013年10月120例外傷性脾破裂手術切除標本進行實驗研究；按照標本破裂時間，把120例標本分成8組，採用常規染色體觀察標本破裂齣血區域邊緣組織學的變化情況，經免疫組織染色後觀察標本破裂齣血區域SMA暘性細胞的錶達規律。結果顯示，損傷時間約6h組的標本有纖維素滲齣，7~12h組有大量中性粒細胞浸潤，13h~1d組分散少量SMA暘性細胞，1~2d組含有的SMA暘性細胞呈網狀分佈，2~3d組含有大量SMA暘性細胞，3~7d組和8~14d組齣現肉芽組織，躍14d組的標本有瘢痕形成，各組標本SMA暘性細胞的數量差異具有統計學意義(P<0.05)。以脾髒標本破裂齣血區域邊緣組織學的變化情況，推斷人脾破裂損傷時間的法醫病理學鑒定方法具有一定的準確性和可靠性。
위관찰화분석법의병이학추단인비파렬손상시간적방법화특점。본문선취2010년6월지2013년10월120예외상성비파렬수술절제표본진행실험연구；안조표본파렬시간，파120례표본분성8조，채용상규염색체관찰표본파렬출혈구역변연조직학적변화정황，경면역조직염색후관찰표본파렬출혈구역SMA양성세포적표체규률。결과현시，손상시간약6h조적표본유섬유소삼출，7~12h조유대량중성립세포침윤，13h~1d조분산소량SMA양성세포，1~2d조함유적SMA양성세포정망상분포，2~3d조함유대량SMA양성세포，3~7d조화8~14d조출현육아조직，약14d조적표본유반흔형성，각조표본SMA양성세포적수량차이구유통계학의의(P<0.05)。이비장표본파렬출혈구역변연조직학적변화정황，추단인비파렬손상시간적법의병이학감정방법구유일정적준학성화가고성。
In order to observe and analyze the methods and characteristics of time estimation of human spleen rupture with forensic pathology, this article selects the surgically resected specimens of 120 cases of trau-matic splenic rupture from June 2010 to October 2013 for experimental research; according to the rupture time, the specimens were divided into 8 groups, histologic changes of rupture bleeding edge were observed by the con-ventional chromosome, and SMA positive cells expression patterns of rupture bleeding area were observed after immunohistochemical staining. The results show: the group with injury time < 6 h has fibrinous exudates, the group with injury time of 7-12 h has a large number of neutrophil infiltration, the group with injury time of 13h-1d is scattered with small amounts of SMA positive cells, the group with injury time of 1-2d has net-like distri-bution of SMA positive cells, the group with injury time of 2-3d contains a lot of SMA positive cells, the group with injury time of 3-7d and the group with injury time 8-14 d have dgranulation tissue, the group with injury time>14d has scarring, and the number difference of each group's SMA positive cells is of statistical significance(P<0.05). Therefore, forensic pathology appraisal method of predicting injury time of human spleen rupture with histologic changes of rupture bleeding edge has certain accuracy and reliability.
