CAJ | 학술논문

目的：建立石菖蒲挥发油β－细辛醚、α－细辛醚和甲基丁香酚的GC－MS测定方法。方法：采用气相色谱－质谱联用法选择离子模式（SIM）检测，DB －1701毛细管色谱柱（60m ×0．25mm ×0．25μm），进样量1μL，分流比为5：1，载气为高纯氦气；电子轰击电离方式，能量70eV，倍增电压1070V ；选择m／z208为β－细辛醚和α－细辛醚的检测离子，m／z178为甲基丁香酚的检测离子。结果：β－细辛醚、α－细辛醚和甲基丁香酚分别在525～5250ng／mL，60～600ng／mL，50～500ng／mL呈良好的线性关系。平均回收率分别为105．5％，103．6％，101．8％。结论：所建立的方法灵敏度高，重现性好，可同时对β－细辛醚、α－细辛醚和甲基丁香酚的含量进行测定。
목적：건립석창포휘발유β－세신미、α－세신미화갑기정향분적GC－MS측정방법。방법：채용기상색보－질보련용법선택리자모식（SIM）검측，DB －1701모세관색보주（60m ×0．25mm ×0．25μm），진양량1μL，분류비위5：1，재기위고순양기；전자굉격전리방식，능량70eV，배증전압1070V ；선택m／z208위β－세신미화α－세신미적검측리자，m／z178위갑기정향분적검측리자。결과：β－세신미、α－세신미화갑기정향분분별재525～5250ng／mL，60～600ng／mL，50～500ng／mL정량호적선성관계。평균회수솔분별위105．5％，103．6％，101．8％。결론：소건립적방법령민도고，중현성호，가동시대β－세신미、α－세신미화갑기정향분적함량진행측정。
Objective:To develop a GC-MS method for determination ofβ-asarone,α-asarone and methyl eugenol in Acorus Tatarinowii Schott volatile oil.Methods:A GC-MS-SIM method was established.The column was DB-1701 capillary column (60 m ×0.25 mm ×0.25μm) .The sample volume was 1μL with a split ratio of 5:1 .The carrier gas was 99.99%high purity Helium.Ionization was performed in electron impact ionization (EI) mode at 70 eV.Selected ions were m/z 208 forβ-asarone,α-asarone and m/z 178 for methyl eugenol.Results:The linear range was 525~5250 ng/ml forβ-asarone, 60~600 ng/ml forα-asarone and 50~500 ng/ml for methyl eugenol.The average recov-eries ofβ-asarone,α-asarone and methyl eugenol were 105.5%, 103.6%and 101.8% respectively.Conclusion:This method is accurate and sensitive, and it can apply to simultaneously determine the concentrations of β-asarone,α-asarone and methyl eugenol in Acorus Tatarinowii Schott volatile oil .