CAJ | 학술논문

目的：建立柱前衍生HPLC法测定瓜蒌皮中精氨酸含量的方法。方法：采用邻苯二甲醛（ OPA ）为衍生化试剂，色谱柱为Eclipse XDB C18（4．6mm ×250mm，5μm）；流动相A为甲醇－乙腈－水（45∶45∶10），流动相B为0．01mol／L磷酸氢二钠－0．01mol／L硼酸钠的缓冲溶液（pH＝8．2），梯度洗脱，流速为1mL／min；检测波长为338nm。结果：该方法可以对瓜蒌皮中精氨酸含量准确定量，其浓度在12～4000mg／L范围内，线性关系良好（R2＝0．9999）。方法精密度、重复性均小于2％，平均加样回收率为102．36％，RSD为1．89％（n＝5）。10批不同产地瓜蒌皮中精氨酸测定结果差异较大，其中，山东（道地产区）瓜蒌皮中精氨酸含量最多。结论：该方法衍生方便、快速，产物单一，测定结果准确、可靠，可用于瓜蒌皮中精氨酸的含量测定。
목적：건립주전연생HPLC법측정과루피중정안산함량적방법。방법：채용린분이갑철（ OPA ）위연생화시제，색보주위Eclipse XDB C18（4．6mm ×250mm，5μm）；류동상A위갑순－을정－수（45∶45∶10），류동상B위0．01mol／L린산경이납－0．01mol／L붕산납적완충용액（pH＝8．2），제도세탈，류속위1mL／min；검측파장위338nm。결과：해방법가이대과루피중정안산함량준학정량，기농도재12～4000mg／L범위내，선성관계량호（R2＝0．9999）。방법정밀도、중복성균소우2％，평균가양회수솔위102．36％，RSD위1．89％（n＝5）。10비불동산지과루피중정안산측정결과차이교대，기중，산동（도지산구）과루피중정안산함량최다。결론：해방법연생방편、쾌속，산물단일，측정결과준학、가고，가용우과루피중정안산적함량측정。
Objective:To establish a pre-column derivatization method of HPLC to determine the constituent of arginine in Trichosanthes pericarpium.Methods:Orthophthalaldehyde ( OPA) was used as a derivatization reagent.Chromato-graphic condition:Eclipse XDB C18 HPLC column, (4.6mm ×250mm,5μm), mobile phase:gradient elution of metha-nol-acetonitrile-water (45∶45∶10) (A) and disodium hydrogen phosphate-sodium borate buffer (ca.0.01 mol/L, pH=8.2) (B), column temperature:30℃, detector wavelength:338nm.Results:The method was simple and accu-rate with good reproducibility and could be used for the quantitative analysis of arginine in Trichosanthes pericarpium.It had a good linear relationship in the scope of 12~4 000mg/L( R2 =0.999 9) .The RSD of the method in precision and repeatability was less than 2%.The average recovery was 102.36%, RSD was 1.89%(n=5).The contents of argi-nine in 10 batches of Trichosanthes pericarpium were different, among which the content of arginine in Shandong ( real estate areas) was the highest.Conclusion:The method is accurate, reliable and sensitive, which can be used for the quantitative analysis of arginine in Trichosanthes pericarpium.