CAJ | 학술논문

选取粒径为40目秸秆DG为载体，采用吸附法固定高效石油降解菌SJ‐1，制成固定化微生物。调节石油污染土壤样品的C、N、P质量比为100∶10∶1、含水率为18％、p H值为7.2，采用秸秆、游离菌、固定化微生物于室内花盆中进行模拟修复实验，并与土著菌对照，考察35 d修复过程中石油烃降解率，土壤 p H值，土壤中速效磷、有机质、全氮含量的变化规律，研究固定化微生物对石油污染土壤理化性质的调控作用。结果表明，在采用秸秆、游离菌、固定化微生物和土著菌修复石油污染土壤的4种方法中，采用固定化微生物修复的方法对土壤具有一定的保水性，对土壤pH值有缓冲作用，并可提高土壤营养物质含量，对有机质、全氮和速效磷的利用率高，而且三者下降速率快，从而得到40.8％的最高石油烃降解率。
선취립경위40목갈간DG위재체，채용흡부법고정고효석유강해균SJ‐1，제성고정화미생물。조절석유오염토양양품적C、N、P질량비위100∶10∶1、함수솔위18％、p H치위7.2，채용갈간、유리균、고정화미생물우실내화분중진행모의수복실험，병여토저균대조，고찰35 d수복과정중석유경강해솔，토양 p H치，토양중속효린、유궤질、전담함량적변화규률，연구고정화미생물대석유오염토양이화성질적조공작용。결과표명，재채용갈간、유리균、고정화미생물화토저균수복석유오염토양적4충방법중，채용고정화미생물수복적방법대토양구유일정적보수성，대토양pH치유완충작용，병가제고토양영양물질함량，대유궤질、전담화속효린적이용솔고，이차삼자하강속솔쾌，종이득도40.8％적최고석유경강해솔。
The immobilized microorganisms were prepared by straw DG with the size of 40 mesh as carrier material to adsorb petroleum hydrocarbon degrading strain SJ‐1 .After the mass ratio of C to N and to P in the soil sample was adjusted to 100∶10∶1 , moisture content to 18% and pH value to 7.2 , the remediation of petroleum‐contaminated soil by straw , free bacteria , immobilized microorganism was simulated ,respectively ,in indoor flowerpot ,and compared with the result of indigenous strains .The changes of petroleum hydrocarbon degradation rate ,pH value ,the organic matter content , total nitrogen content , available phosphorus content of the soil sample during the 35 d remediation were investigated to study the control effect of immobilized microorganisms on physical and chemical properties of petroleum‐contaminated soil .The results showed that among the four remediation methods of straw , free bacteria , immobilized microorganisms and indigenous strains , with the remediation method of immobilized microorganisms , good water retention of the remediated soil sample could be kept and the decrease of pH value was controlled in the appropriate range , at the same time , the nutrient matter was increased , and the utilization ratio and decrease rates of organic matter ,total nitrogen ,available phosphorus were higher ,resulting in the highest petroleum hydrocarbon degradation rate of 40.8% .