CAJ | 학술논문

裂解性噬菌体能够特异性裂解细菌，本研究分析了一株从自然界中分离的宽宿主谱裂解性大肠杆菌噬菌体的种属和进化关系。前期用Adams双层平板琼脂法从猪场污水中分离纯化出一株裂解性噬菌体vB_EcoM_JS09，裂解谱分析能够裂解禽致病性大肠杆菌和产肠毒素大肠杆菌。扩增分析gene18、gene 23序列，并根据gp18和gp23氨基酸序列构建系统进化树，分析JS09的遗传进化关系。结果表明噬菌体vB_EcoM_JS09基因组为dsDNA，属于有尾噬菌体目、肌尾噬菌体科、T4-like噬菌体、T-evens亚群。其gp18氨基酸序列与大肠杆菌噬菌体RB69同源性最高，为100%；gp23氨基酸序列与大肠杆菌T4噬菌体同源性最高为96%。该结果为禽致病性大肠杆菌和产肠毒素大肠杆菌的防控提供了生物学基础。
렬해성서균체능구특이성렬해세균，본연구분석료일주종자연계중분리적관숙주보렬해성대장간균서균체적충속화진화관계。전기용Adams쌍층평판경지법종저장오수중분리순화출일주렬해성서균체vB_EcoM_JS09，렬해보분석능구렬해금치병성대장간균화산장독소대장간균。확증분석gene18、gene 23서렬，병근거gp18화gp23안기산서렬구건계통진화수，분석JS09적유전진화관계。결과표명서균체vB_EcoM_JS09기인조위dsDNA，속우유미서균체목、기미서균체과、T4-like서균체、T-evens아군。기gp18안기산서렬여대장간균서균체RB69동원성최고，위100%；gp23안기산서렬여대장간균T4서균체동원성최고위96%。해결과위금치병성대장간균화산장독소대장간균적방공제공료생물학기출。
Lytic bacteriophage (phage) can kill bacteria. This study analyzed the classification and phylogeny of a broad-host-range lytic coliphage isolated from the nature. In a previous study, a strain of phage vB_EcoM_JS09 was isolated by using Adams double-layer agar plate method and purified from the sewage of a swine farm. The genome was extracted through phenol chloroform isopropanol method, then treated with DNase I, RNase A and Mung Bean Nuclease. The conserved tail sheath gene 18 and major capsid protein gene 23 of T4-like phages were amplified using the reported primers and JS09 as a template and their deduced amino acid sequences were used for phylogenetic tree analysis. Results showed that lytic coliphage JS09 had the genome of double stranded DNA, belonging to the Caudovirales, Myoviridae, T4-like phage, T-even subgroups. The homology of amino acid sequence of gp18 was 100%with E.coli phage RB69 while that of gp23 shared 96%identity to E.coli T4 phage. The results provide a good foundation for the biology of Avian pathogenic Escherichiacoli and enterotoxigenic Escherichiacoli prevertion Cuel control.