CAJ | 학술논문

以赤子爱胜蚓为生物载体，在蚯蚓饲料中添加亚硒酸钠进行富硒蚯蚓培养试验，利用蚯蚓的生物富集作用将无机硒转化为有机硒。以存活率、硒含量为优化指标，研究5种硒浓度（20、40、60、80、100 mg·kg-1）和4种培养时间（第15、30、45、60 d）对蚯蚓生长和硒含量的影响规律，筛选存活率高、硒含量高、培养时间短的富硒蚯蚓培养方法。试验结果表明：蚯蚓饲料中亚硒酸钠含量越高、培养时间越长，蚯蚓的存活率越低，硒含量越高，且当硒添加量为80 mg·kg-1，培养时间为45 d时，培养条件最优，此时蚯蚓体内硒含量可达33.25 mg·kg-1。
이적자애성인위생물재체，재구인사료중첨가아서산납진행부서구인배양시험，이용구인적생물부집작용장무궤서전화위유궤서。이존활솔、서함량위우화지표，연구5충서농도（20、40、60、80、100 mg·kg-1）화4충배양시간（제15、30、45、60 d）대구인생장화서함량적영향규률，사선존활솔고、서함량고、배양시간단적부서구인배양방법。시험결과표명：구인사료중아서산납함량월고、배양시간월장，구인적존활솔월저，서함량월고，차당서첨가량위80 mg·kg-1，배양시간위45 d시，배양조건최우，차시구인체내서함량가체33.25 mg·kg-1。
As a bio-carrier, Eisenia fetida was fed with cow dung that added with sodium selenite in order to transfer inorganic selenium(Se) into organic selenium. Targeting on survival rate and selenium content, the effects of five Se concentrations(0, 20, 40, 60, 80, 100 mg·kg-1) and four cultivation periods(15, 30, 45, 60 d)on earthworm growth and Se contents were investigated. The cultivation method with high sur-vival rate, high Se content of earthworm and short breeding time would be screened out. The experimental results showed that the earthworm survival rate decreased and the Se content in earthworm increased with the increase of Se application and the extension of breeding time. The most optimum method was screened out when the Se concentration was 80 mg·kg-1 and the cultivation period was 45 days, Se content in earth-worm was up to 33.25 mg·kg-1.