CAJ | 학술논문

通过比较293T细胞的接种密度、质粒DNA的量以及 CO2浓度对磷酸钙转染方法的影响，确定最佳的转染条件。以各单因素试验的最适条件为依据，设计三因素三水平的正交试验。结果表明，磷酸钙介导质粒DNA转染，当293T细胞的接种密度为5×105/mL、质粒 DNA 的量为30μg、细胞培养环境 CO2浓度为3%时，转染效果最佳。
통과비교293T세포적접충밀도、질립DNA적량이급 CO2농도대린산개전염방법적영향，학정최가적전염조건。이각단인소시험적최괄조건위의거，설계삼인소삼수평적정교시험。결과표명，린산개개도질립DNA전염，당293T세포적접충밀도위5×105/mL、질립 DNA 적량위30μg、세포배양배경 CO2농도위3%시，전염효과최가。
In this study,the effects of seeding density of 293T cells,as well as the amount of plasmid DNA and the concentration of CO2 in culture environment,on the efficiency of calcium phosphate transfec—tion were compared,optimum transfection condition was confirmed.On the basis of optimum condition obtained from single factor experiments,orthogonal tests in three levels of three factors were designed and performed.The results showed that the highest transfection efficiency was obtained when the seeding den—sity of 293T cells was 5×105/mL,the amount of plasmid DNA was 30μg and the concentration of CO2 in culture environment was 3%.