CAJ | 학술논문

以银杏木质素-碳水化合物复合体( LCC)为原料，通过洗脱支架的方法制备医用多孔生物载体，并利用红外光谱、液相色谱、核磁共振波谱及扫描电子显微镜对该生物载体的主要成分含量、化学结构以及表面形态进行分析。结果表明：以银杏LCC为原料可以制备性能优良的生物载体。该载体不仅含有愈创木基型苯丙烷结构单元，而且糖组分中对人体肝细胞有较高生物相容性的半乳糖和甘露糖分别占3．30%和21.94%。制备的多孔生物载体孔隙率可达60%以上，平均孔径约为130μm。用多孔生物载体进行人体肝细胞培养，发现人体肝细胞能够成功地在银杏LCC多孔生物载体上生长，且细胞增殖较快，白蛋白分泌和葡萄糖代谢活性的测定结果表明肝细胞具有较高的代谢活性。研究结果表明银杏LCC多孔生物载体具有良好的生物相容性。
이은행목질소-탄수화합물복합체( LCC)위원료，통과세탈지가적방법제비의용다공생물재체，병이용홍외광보、액상색보、핵자공진파보급소묘전자현미경대해생물재체적주요성분함량、화학결구이급표면형태진행분석。결과표명：이은행LCC위원료가이제비성능우량적생물재체。해재체불부함유유창목기형분병완결구단원，이차당조분중대인체간세포유교고생물상용성적반유당화감로당분별점3．30%화21.94%。제비적다공생물재체공극솔가체60%이상，평균공경약위130μm。용다공생물재체진행인체간세포배양，발현인체간세포능구성공지재은행LCC다공생물재체상생장，차세포증식교쾌，백단백분비화포도당대사활성적측정결과표명간세포구유교고적대사활성。연구결과표명은행LCC다공생물재체구유량호적생물상용성。
A porous biological carrier was prepared by the method of eluting stent with lignin-carbohydrate complexes ( LCC ) isolated from ginkgo wood. Fourier transform infrared spectrometer(FT-IR), high performance liquid chromatograph(HPLC), nuclear magnetic resonance ( NMR ) spectroscopy and scanning electron microscope ( SEM ) were applied to elucidate the composition, chemical structure and morphology of the biological carrier. The results indicate that a biological carrier with excellent performance can be prepared with gingko LCC as raw material. Not only the guaiacyl lignin subunits existe in this biological carrier, but also the galactose and mannose with high biological compatibility to human hepatocytes are found to be 3. 30% and 21. 94%, respectively. The porosity of prepared porous scaffold is more than 60% with average pore size of 130μm. The carriers were used to culture human hepatocytes. The results show that human hepatocytes can grow successfully on carriers. Furthermore, the human hepatocytes on carriers proliferate rapidly and show a high metabolic activity by determination of human albumin and glucose metabolism. These interesting results indicate that porous biological carrier prepared with gingko LCC have an excellent biocompatibility.