CAJ | 학술논문

以戊二醛交联胶原纤维吸附剂( CFA)为填料,对两种结构相近的单糖基黄酮苷类化合物(染料木苷和黄芩苷)进行了柱层析分离。结果表明,通过改变乙醇水溶液的浓度,可以调节CFA对染料木苷和黄芩苷的吸附选择性；当CFA的用量为6 g,层析柱的高径比为10∶1时,采用100%、90%和70%乙醇水溶液进行分步洗脱,染料木苷和黄芩苷能够得到分离。分离所得染料木苷和黄芩苷的纯度分别为98%和97%,回收率分别为99.35%和96.52%。
이무이철교련효원섬유흡부제( CFA)위전료,대량충결구상근적단당기황동감류화합물(염료목감화황금감)진행료주층석분리。결과표명,통과개변을순수용액적농도,가이조절CFA대염료목감화황금감적흡부선택성；당CFA적용량위6 g,층석주적고경비위10∶1시,채용100%、90%화70%을순수용액진행분보세탈,염료목감화황금감능구득도분리。분리소득염료목감화황금감적순도분별위98%화97%,회수솔분별위99.35%화96.52%。
The collagenous fiber cross-linked by glutaraldehyde was used as an adsorbent ( CFA) for chromatographic separation of single glucosyl flavonoid glycosides. Genistin and baicalin with similar molecular structure were chosen as the representatives of flavonoid glycosides in the experiments is this paper. It was found that the adsorptive affinity and selectivity of CFA to genistin and baicalin could be adjusted by ethanol concentration in aqueous solution. In column chromatographic separation, where the CFA dosage was 6 g and the ratio of column height to diameter was 10∶1, genistin and baicalin were well separated by stepwise elution of 100 %-90 %-70 % ethanol aqueous solutions. The purities of the obtained genistin and baicalin were 98 % and 97 %, and their recovery rates were 99. 35% and 96. 52 %, respectively.