南京晓庄学院学报
南京曉莊學院學報
남경효장학원학보
JOURNAL OF NANJING XIAOZHUANG COLLEGE
2014年
6期
50-53
,共4页
冠心宁注射液%HPLC-RID%果糖%葡萄糖%蔗糖%含量测定
冠心寧註射液%HPLC-RID%果糖%葡萄糖%蔗糖%含量測定
관심저주사액%HPLC-RID%과당%포도당%자당%함량측정
Guan-Xin-Ning Injection%HPLC-RID%fructose%glucose%sucrose%determination
目的：：建立高效液相色谱联用示差折光检测器测定冠心宁注射液中果糖、葡萄糖和蔗糖含量的方法.方法：Hanbon Lichrospher NH2分析色谱柱(5μm,4.6×250 mm),Welchrom 氨基保护柱(5μm,10 mm ×4.6 mm),流动相为乙腈—水(70∶30),柱温：30℃,流速：1.0 ml/min,示差折光检测器设定温度：35℃.结果：回归方程A果糖=85539C+677.38,r=0.9999,A葡萄糖=84012 C+575.37,r=1,A蔗糖=187714C+504.15,r=1(n=6),果糖浓度在33.72~1079μg/ml之间、葡萄糖浓度在32.22~1031μg/ml之间以及蔗糖浓度在16.19~518μg/ml之间线性关系良好；平均回收率分别为100.1%、100.6%和99.3%, RSD分别为1.99、2.35和2.84%( n=6)；注射液中果糖、葡萄糖和蔗糖的总含量占固含物的55.03%.结论：该方法简便快速、准确可靠,可用于冠心宁注射液中果糖、葡萄糖及蔗糖的含量测定.
目的：：建立高效液相色譜聯用示差摺光檢測器測定冠心寧註射液中果糖、葡萄糖和蔗糖含量的方法.方法：Hanbon Lichrospher NH2分析色譜柱(5μm,4.6×250 mm),Welchrom 氨基保護柱(5μm,10 mm ×4.6 mm),流動相為乙腈—水(70∶30),柱溫：30℃,流速：1.0 ml/min,示差摺光檢測器設定溫度：35℃.結果：迴歸方程A果糖=85539C+677.38,r=0.9999,A葡萄糖=84012 C+575.37,r=1,A蔗糖=187714C+504.15,r=1(n=6),果糖濃度在33.72~1079μg/ml之間、葡萄糖濃度在32.22~1031μg/ml之間以及蔗糖濃度在16.19~518μg/ml之間線性關繫良好；平均迴收率分彆為100.1%、100.6%和99.3%, RSD分彆為1.99、2.35和2.84%( n=6)；註射液中果糖、葡萄糖和蔗糖的總含量佔固含物的55.03%.結論：該方法簡便快速、準確可靠,可用于冠心寧註射液中果糖、葡萄糖及蔗糖的含量測定.
목적：：건립고효액상색보련용시차절광검측기측정관심저주사액중과당、포도당화자당함량적방법.방법：Hanbon Lichrospher NH2분석색보주(5μm,4.6×250 mm),Welchrom 안기보호주(5μm,10 mm ×4.6 mm),류동상위을정—수(70∶30),주온：30℃,류속：1.0 ml/min,시차절광검측기설정온도：35℃.결과：회귀방정A과당=85539C+677.38,r=0.9999,A포도당=84012 C+575.37,r=1,A자당=187714C+504.15,r=1(n=6),과당농도재33.72~1079μg/ml지간、포도당농도재32.22~1031μg/ml지간이급자당농도재16.19~518μg/ml지간선성관계량호；평균회수솔분별위100.1%、100.6%화99.3%, RSD분별위1.99、2.35화2.84%( n=6)；주사액중과당、포도당화자당적총함량점고함물적55.03%.결론：해방법간편쾌속、준학가고,가용우관심저주사액중과당、포도당급자당적함량측정.
The present study is to establish the HPLC-RID method which is used to determine the contents of fruc-tose, glucose and sucrose in Guan-Xin-Ning ( GXN ) injection. The chromatographic conditions were as follows:Hanbon Lichrospher NH2 column(5 μm,250 × 4. 6 mm) and Welchrom NH2 protection column (5 μm,10 mm × 4. 6 mm)with the temperature of 30 ℃, acetonitrile-water(70∶30,V/V) as a mobile phase with the flow rate of 1. 0 mL/min, refractive index detector ( RID) at 35 ℃. The results showed that the regression equations of fruc-tose, glucose and sucrose were separately Afructose=85 539C+677. 38,r=0. 999 9,Aglucose=84 012C+575. 37,r=1 and Asucrose=187 714C+504. 15,r=1(n=6). Their linear ranges were 33. 72~1 079 μg/ml, 32. 22~1 031μg/ml and 16. 19~518 μg/ml, respectively. The average recoveries and RSDs of the three kinds of sugar compo-sitions were 100. 1%, 100. 6%, 99. 3% and 1. 99, 2. 35, 2. 84% (n=6) in turn. The total content of fructose, glucose and sucrose accounted for 55. 03% of solid substance in GXN injection. It is concluded that the approach is simple, rapid, accurate and reliable, which can be applied as the determinations of fructose, glucose and sucrose in GXN injection.
