CAJ | 학술논문

化学农药的广泛使用，不可避免的产生了农药残留问题.蔬菜作为人类食物之一，其农药残留问题尤其重要.本文采用饱和硫酸铵盐析法提纯噻虫啉，并合成了CdS纳米晶，制备了具有稳定且强电致化学发光的CdS纳米晶膜，在纳米晶膜上建立了农药残留物噻虫啉含量的电化学发光免疫检测方法.该方法利用具有优良导电性的石墨烯作为抗原标记物，实现了噻虫啉的灵敏检测.标记上石墨烯的噻虫啉与纳米晶膜表面的抗体免疫结合后引起纳米晶膜发光的增强，发光强度的增加随着噻虫啉的含量的增加而增大.发光强度的对数与噻虫啉浓度的对数在0.1~10 pg·mL-1范围内呈线性关系(R=0.99).在S/N比为3时，检测限为0.1 pg·mL-1.利用该方法检测实际蔬菜样品中的农药残留量，取得了令人满意的结果.鉴于石墨烯良好的可修饰性，其作为标记物在其他免疫反应、DNA杂化分析中具有十分广阔的前景.
화학농약적엄범사용，불가피면적산생료농약잔류문제.소채작위인류식물지일，기농약잔류문제우기중요.본문채용포화류산안염석법제순새충람，병합성료CdS납미정，제비료구유은정차강전치화학발광적CdS납미정막，재납미정막상건립료농약잔류물새충람함량적전화학발광면역검측방법.해방법이용구유우량도전성적석묵희작위항원표기물，실현료새충람적령민검측.표기상석묵희적새충람여납미정막표면적항체면역결합후인기납미정막발광적증강，발광강도적증가수착새충람적함량적증가이증대.발광강도적대수여새충람농도적대수재0.1~10 pg·mL-1범위내정선성관계(R=0.99).재S/N비위3시，검측한위0.1 pg·mL-1.이용해방법검측실제소채양품중적농약잔류량，취득료령인만의적결과.감우석묵희량호적가수식성，기작위표기물재기타면역반응、DNA잡화분석중구유십분엄활적전경.
The problem of the pesticide residue results from a wide use of chemical pesticides. It is of great impor-tance to deal with the problem of pesticide residues in vegetables. In this paper, an electrochemiluminescence im-munoassay method was established to detect the thiacloprid content of pesticide residues. This method used CdS nanocrystals ( NCs) and graphene as ECL emitters and antigen Label respectively. When thiacloprid ( the antigen) labeled with graphene was bound to antibody on the CdS NCs film through the formation of immunocomplex, ECL e-mission from CdS NCs film was greatly enhanced. The ECL increment (ΔI) increased with the concentration of thi-acloprid . The logarithm of the concentration of thiacloprid is linearly related to the logarithm of ECL increment with-in the range of 0. 1~10 pg. mL-1(R = 0. 99). At a S/N ratio of 3, the detection limit was 0. 1 pg. mL-1. Satis-factory results were obtained when this method was applied to the detection of pesticide residues in real samples. Graphene, easily being modified, has a very broad prospect as a label to be used in other immunoassay as well as in DNA hybridization analysis.