CAJ | 학술논문

对杜仲叶渣固态发酵制备有机肥的工艺进行了研究。通过单因素分析了4个主要因素：菌种组合、液料比、葡萄糖添加量和发酵时间对杜仲叶渣中腐殖酸质量分数的影响。在单因素试验的基础上，通过正交试验优化了杜仲叶渣固态发酵工艺条件，并考察了所制备的有机肥的毒性。结果表明，杜仲叶渣最佳固态发酵工艺条件为：液料比2.0∶1(mL∶ g)，葡萄糖添加量1.5%，接入白腐菌和康宁木霉复合菌种，发酵6 d。此条件下，杜仲叶渣中的腐殖酸质量分数可达38.31%。毒性试验结果表明，上海青种子的发芽率均达到90%以上，证明制备的有机肥无毒。
대두중협사고태발효제비유궤비적공예진행료연구。통과단인소분석료4개주요인소：균충조합、액료비、포도당첨가량화발효시간대두중협사중부식산질량분수적영향。재단인소시험적기출상，통과정교시험우화료두중협사고태발효공예조건，병고찰료소제비적유궤비적독성。결과표명，두중협사최가고태발효공예조건위：액료비2.0∶1(mL∶ g)，포도당첨가량1.5%，접입백부균화강저목매복합균충，발효6 d。차조건하，두중협사중적부식산질량분수가체38.31%。독성시험결과표명，상해청충자적발아솔균체도90%이상，증명제비적유궤비무독。
The preparation process of organic fertilizer by solid-state fermentation of leaves residual of Eucommia ulmoides Oliv. was studied. Effects of four main factors, including bacteria combination, ratio of liquor to material, additive amount of glucose and fermentation time on the mass fraction of humic acid, were analyzed by using single factor method. Based on the single factor test, the process conditions of solid-state fermentation of Eucommia unmolds Oliv. were optimized by the orthogonal experiments method, and toxicity of the vested organic fertilizer was tested. The results showed that the optimum condition were the ratio of liquor to material 2. 0∶1, additive amount of glucose 1. 5%, and inoculating the composite bacteria of white rot fungus and Trichoderma koningii to the Eucommia ulmoides Oliv. , and fermentation time 6 days. In this experimental condition, the mass fraction of humic acid in the leaves residual of Eucommia ulmoides Oliv. could reach 38. 31%. The results of toxicity test showed that the germination rate of brassica chinensis were all higher than 90%. It proved that the organic fertilizer prepared by leaves residual of Eucommia ulmoides Oliv. was nontoxic.