CAJ | 학술논문

通过大孔吸附树脂-C18反相硅胶柱层析-正相硅胶柱层析联用技术，对杜仲总木脂素及松脂醇二葡萄糖苷(PDG)进行纯化研究。结果表明，含总木脂素23.33%和松脂醇二葡萄糖苷粗品0.9605%的杜仲提取液经AB-8树脂一次纯化，可以制得71.61%的杜仲总木脂素有效部位以及8.070%的松脂醇二葡萄糖苷粗品，再经C18反相硅胶柱层析纯化，得到53.12%的松脂醇二葡萄糖苷。最后通过正相硅胶柱层析进一步纯化，得到91.50%的松脂醇二葡萄糖苷产品。
통과대공흡부수지-C18반상규효주층석-정상규효주층석련용기술，대두중총목지소급송지순이포도당감(PDG)진행순화연구。결과표명，함총목지소23.33%화송지순이포도당감조품0.9605%적두중제취액경AB-8수지일차순화，가이제득71.61%적두중총목지소유효부위이급8.070%적송지순이포도당감조품，재경C18반상규효주층석순화，득도53.12%적송지순이포도당감。최후통과정상규효주층석진일보순화，득도91.50%적송지순이포도당감산품。
Purification of lignans and pinoresinol diglucoside ( PDG ) from Eucommia bark extract was studied by a combined method of macroporous adsorption resin-C18 column chromatography and silica gel column chromatography. The results indicated that AB-8 resin was the best one on the adsorption and desorption rates by comparing with the other 3 kinds of resins. The purity of effective part of lignans and the crude product of PDG were respectively from 23. 33% to 71. 61% and 0. 960 5% to 8. 070 %through AB-8 resin once. Then the purity of PDG increased to 53. 12 % after C18 purification and finally reached 91. 50 % after purifying by normal phase silica gel column chromatography.