海军医学杂志
海軍醫學雜誌
해군의학잡지
JOURNAL OF NAVY MEDICINE
2014年
6期
430-432
,共3页
冯旭%汪家春%赵龙%陈虹%刁琢%储智勇
馮旭%汪傢春%趙龍%陳虹%刁琢%儲智勇
풍욱%왕가춘%조룡%진홍%조탁%저지용
冬虫夏草口服液%微生物限度检查%方法验证
鼕蟲夏草口服液%微生物限度檢查%方法驗證
동충하초구복액%미생물한도검사%방법험증
Cordyceps sinensis oral liquid%Microbial limit test%Verification of the method
目的:建立冬虫夏草口服液微生物限度检查方法,并对方法进行验证。方法采用常规法,通过大肠埃希菌、金黄色葡萄球菌、枯草芽孢杆菌、白色念球菌、黑曲霉5种阳性菌回收率试验进行细菌、霉菌和酵母菌计数方法的验证,同时对控制菌检查方法进行验证。结果采用常规法计数细菌、霉菌及酵母菌时,各试验菌回收率在70%以上;控制菌检查中,各阳性试验菌均检出,阴性对照无菌生长。结论可以采用常规法进行冬虫夏草口服液的微生物限度检查。
目的:建立鼕蟲夏草口服液微生物限度檢查方法,併對方法進行驗證。方法採用常規法,通過大腸埃希菌、金黃色葡萄毬菌、枯草芽孢桿菌、白色唸毬菌、黑麯黴5種暘性菌迴收率試驗進行細菌、黴菌和酵母菌計數方法的驗證,同時對控製菌檢查方法進行驗證。結果採用常規法計數細菌、黴菌及酵母菌時,各試驗菌迴收率在70%以上;控製菌檢查中,各暘性試驗菌均檢齣,陰性對照無菌生長。結論可以採用常規法進行鼕蟲夏草口服液的微生物限度檢查。
목적:건입동충하초구복액미생물한도검사방법,병대방법진행험증。방법채용상규법,통과대장애희균、금황색포도구균、고초아포간균、백색념구균、흑곡매5충양성균회수솔시험진행세균、매균화효모균계수방법적험증,동시대공제균검사방법진행험증。결과채용상규법계수세균、매균급효모균시,각시험균회수솔재70%이상;공제균검사중,각양성시험균균검출,음성대조무균생장。결론가이채용상규법진행동충하초구복액적미생물한도검사。
Objective To establish a method of microbial limit test for Cordyceps sinensis oral liquid and to verify the validity of the testing method .Methods Conventional methods were used to verify the counting methods for microbial , fungus and yeast counts through the measurement of the recovery rates of 5 positive bacteria in the Cordyceps sinensis oral liquid.At the same time, the detection method for the control bacteria in the Cordyceps sinensis oral liquid was also verified .Results When conventional detection methods were used, the recovery rates of various bacteria were over 70%.In the detection of the control bacteria , positive test bacteria were all detected, while no bacterial growth was detected in the control .Conclusion Conventional methods could be used for the detection of microbial limit in the Cordyceps sinensis oral liquid.