CAJ | 학술논문

目的：研究细胞色素P4502C9基因（CYP2C9）和维生素K环氧化物还原酶复合物1基因（VKORC1）在华法令抗凝治疗患者的多态性分布，并探讨其对抗凝剂量的影响。方法：收集74例服用华法令抗凝治疗病人的外周血，测定其凝血酶原时间国际标准化比值（INR）和CYP2C9、VKORC1基因类型，探讨基因多态性的分布特点，以及华法令维持剂量与基因多态性的关系。结果：CYP2C9基因分布主要为野生型，突变型较少，抗凝治疗的维持剂量野生型组与突变组无明显差别。 VKORC1基因型的分布AA型为主，AG型较少，GG型未见，抗凝治疗的维持剂量AG组明显高于AA组。结论：CYP2C9、VKORC1基因在中国汉族人群中具有遗传多态性， VKORC1基因的多态性在华法令抗凝治疗中具有显著意义。
목적：연구세포색소P4502C9기인（CYP2C9）화유생소K배양화물환원매복합물1기인（VKORC1）재화법령항응치료환자적다태성분포，병탐토기대항응제량적영향。방법：수집74례복용화법령항응치료병인적외주혈，측정기응혈매원시간국제표준화비치（INR）화CYP2C9、VKORC1기인류형，탐토기인다태성적분포특점，이급화법령유지제량여기인다태성적관계。결과：CYP2C9기인분포주요위야생형，돌변형교소，항응치료적유지제량야생형조여돌변조무명현차별。 VKORC1기인형적분포AA형위주，AG형교소，GG형미견，항응치료적유지제량AG조명현고우AA조。결론：CYP2C9、VKORC1기인재중국한족인군중구유유전다태성， VKORC1기인적다태성재화법령항응치료중구유현저의의。
Objective: To investigate the polymorphism distribution and characteristics of cytochrome P450 2C9 (CYP2C9) and vitamin K epoxide reductase complex (VKORC1) in Han population, as well as the relationship between each polymorphism and warfarin anticoagulation dose. Methods: Seventy-four patients in Han population with stable anticoagulation taking warfarin were recruited. Their CYP2C9 and VKORC1 genotypes were tested by pyrosequencing, and their international normalized ratios (INR) were obtained. Re_sults: Most of the patients in our study had wild type CYP2C9, with similar warfarin doseage to those of mutated type. For VKORC1 genotype, most patients with wild type AA had lower warfarin doseage than those with the mutated variant. Conclusion: The genetic polymorphisms of CYP2C9 and VKORC1 present in Han population, and the VKORC1 genotypes could affect individualized warfarin dose in anticoagulation.