CAJ | 학술논문

目的：探讨实验室常用的人胰腺癌细胞株BXPC-3的有效转染方法及最佳转染参数。方法通过脂质体法和电穿孔法将表达绿色荧光蛋白的质粒（ pGFP-N3）导入人胰腺癌细胞株BXPC-3中，24 h后观察并比较细胞的存活率及瞬转效率，确定最佳转染参数。结果常规脂质体法BXPC-3细胞瞬转阳性率为1．36％，细胞存活率为87．45％；而电穿孔法在电压120 V电击时程70μs条件下，BXPC-3细胞瞬转阳性率为54．78％，细胞存活率为64．01％，2种转染方法的瞬转阳性率差异有统计学意义（P＜0．05）。结论转染BXPC-3细胞，电穿孔法可取得比常规脂质体法更高的转染效率。通过条件的优化，电穿孔法可以用于某些脂质体法难以有效转染的细胞株。
목적：탐토실험실상용적인이선암세포주BXPC-3적유효전염방법급최가전염삼수。방법통과지질체법화전천공법장표체록색형광단백적질립（ pGFP-N3）도입인이선암세포주BXPC-3중，24 h후관찰병비교세포적존활솔급순전효솔，학정최가전염삼수。결과상규지질체법BXPC-3세포순전양성솔위1．36％，세포존활솔위87．45％；이전천공법재전압120 V전격시정70μs조건하，BXPC-3세포순전양성솔위54．78％，세포존활솔위64．01％，2충전염방법적순전양성솔차이유통계학의의（P＜0．05）。결론전염BXPC-3세포，전천공법가취득비상규지질체법경고적전염효솔。통과조건적우화，전천공법가이용우모사지질체법난이유효전염적세포주。
Objective To investigate transfection methods and conditions for human pancreatic cancer cell line BXPC -3 and related optimal transfection data .Methods The plasm pGFP-N3 was transfected into BXPC-3 cells respectively by lipofectamine 2000 and electroporation .Then, survival and transient transfection rates of the cells were observed and compared after 24 hours, so as to i-dentify the optimal transfection data .Results The positive BXPC-3 transient transfection efficiency by lipofectamine 2 000 was 1.36%, with the cell survival rate of 87.45%, while the transient transfection efficiency by electroporation with the pulse length of 70μs and e-lectric shock of 120 V was 54.78%, with the cell survival rate of 64.01%.Statistics indicated that there were significant differences in the transient transfection rates, when comparisons were made between the 2 transient transfection methods(P<0.05).Conclusion In the transfection of BXPC-3, electroporation could achieve higher transfection rates than the conventional lipofectamine .Through optimi-zation of transfection conditions , electroporation could effectively transfect certain cell strains , which were hard to be transfected by lipo-fectamine.