CAJ | 학술논문

目的：用高效液相色谱法测定不同来源的莪术中3种姜黄素成分，比较姜黄素（cur_cumin，Ⅰ）、去甲氧基姜黄素（demethoxycurcumin，Ⅱ）和双去甲氧基姜黄素（bisdemethoxycurcum_in，Ⅲ）的含量差异。方法：采用Kromasil色谱柱,流动相为乙腈-水（0.5%冰乙酸）,流速为1 mL· min-1，检测波长为425 nm。结果：姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的线性范围分别为0.04~0.16μg （r=0.9991）、0.037~0.150μg （r=0.9990）、0.00015~0.00062μg （r=0.9996）；平均回收率（n=9）分别为103.32%、100.32%、100.06%。结论：不同来源的莪术中姜黄素类成分的含量存在着明显的差异。
목적：용고효액상색보법측정불동래원적아술중3충강황소성분，비교강황소（cur_cumin，Ⅰ）、거갑양기강황소（demethoxycurcumin，Ⅱ）화쌍거갑양기강황소（bisdemethoxycurcum_in，Ⅲ）적함량차이。방법：채용Kromasil색보주,류동상위을정-수（0.5%빙을산）,류속위1 mL· min-1，검측파장위425 nm。결과：강황소、거갑양기강황소、쌍거갑양기강황소적선성범위분별위0.04~0.16μg （r=0.9991）、0.037~0.150μg （r=0.9990）、0.00015~0.00062μg （r=0.9996）；평균회수솔（n=9）분별위103.32%、100.32%、100.06%。결론：불동래원적아술중강황소류성분적함량존재착명현적차이。
Objective: To research on the content differences of curcuminoids including curcumin, demethoxycurcumin and bisdemethoxycurcumin in Rhizoma Curcumae from different sources. Methods: A reversed-phase HPLC system consisting of an ODS column and a mixture of acetonitrle-water (with 0.5%acetic acid) as the mobile phase was used. The flow rate was 1.0 mL·min-1 and the detection was effected at 425 nm. Results: The calibration curves of curcumin, demethoxycurcumin and bisdemethoxycurcumin were linear in the range of 0.04~0.16μg (r=0.9991), 0.037~0.150μg (r=0.9990), 0.00015~0.00062μg (r=0.9996), respectively; The average recoveries (n=9) were 103.32%, 100.32% and 100.06%, respectively. Conclusion: The results showed that the contents of curcuminoids vary with sample source.