CAJ | 학술논문

目的：探讨热毒宁（RDN）对呼吸道合胞病毒（RSV）感染哮喘加重小鼠气道炎症的影响。方法：雌性BALB/c小鼠32只，随机分成4组，分别为对照组、鸡卵白蛋白（OVA）组、OVA/RSV组、OVA/RSV/RDN组。无创肺功能检测各组小鼠气道反应性；HE染色观察肺部炎症变化；小鼠支气管肺泡灌洗液（BALF）中炎性细胞计数并分类；ELISA法检测BALF中IL-4、IL-5、IL-13、IFN-γ含量。结果：RSV感染能够加重哮喘小鼠气道炎症与气道高反应性， RDN抑制RSV感染哮喘加重小鼠气道高反应性（P<0.05）；RDN显著减少RSV感染哮喘小鼠BALF 中IL-4、IL-5、IL-13、IFN-γ含量。结论：RDN可以减轻RSV感染的小鼠气道炎症反应，抑制气道高反应性。
목적：탐토열독저（RDN）대호흡도합포병독（RSV）감염효천가중소서기도염증적영향。방법：자성BALB/c소서32지，수궤분성4조，분별위대조조、계란백단백（OVA）조、OVA/RSV조、OVA/RSV/RDN조。무창폐공능검측각조소서기도반응성；HE염색관찰폐부염증변화；소서지기관폐포관세액（BALF）중염성세포계수병분류；ELISA법검측BALF중IL-4、IL-5、IL-13、IFN-γ함량。결과：RSV감염능구가중효천소서기도염증여기도고반응성， RDN억제RSV감염효천가중소서기도고반응성（P<0.05）；RDN현저감소RSV감염효천소서BALF 중IL-4、IL-5、IL-13、IFN-γ함량。결론：RDN가이감경RSV감염적소서기도염증반응，억제기도고반응성。
Objective: To evaluate the effect of Reduning (RDN) on respiratory syncytial virus (RSV)-induced airway inflammation and asthma exacerbation in Balb/c mice. Methods: Thirty-two female mice were randomly divided into 4 groups (n=8 for each group): control group, OVA (ovalbumin) group, OVA/RSV group and OVA/RSV/RDN group. Airway responsiveness was assessed using a whole body plethysmog_raphy. Lung histopathological changes were evaluated by HE stain. Cells in bronchoalveolar lavage fluid (BALF) were counted and classified, the supernatants of the BALF were used for detection of interleukin (IL) -4, IL-5, IL-13 and interferon (IFN)-γ. Results: RSV infection induced airway inflammation and air_way hyper-responsiveness (AHR) exacerbation. Administration of RDN remarkably inhibited airway inflam_mation and AHR. IFN-γ, IL-4, IL-5 and IL-13 levels in BALF decreased in OVA/RSV/RDN group com_pared with OVA/RSV group (P<0.01, respectively). Conclusion: RDN might suppress airway inflammation and AHR in RSV infected asthmatic mice.