CAJ | 학술논문

2011年7月,福建省晋江某养殖场养殖的斜带石斑鱼( Epinephelus coioides )发病,病鱼体重3~10 g,症状为鱼体色变深,拒食,离群缓慢游动,背鳍下方局部鳞片脱落、表皮溃烂,肝脏点状发红。2011年7月从濒死病鱼肝脏、肾脏中分离到2株细菌,分别命名为201107-I与201107-II,经生理、生化指标和16S rRNA 基因鉴定,这两株均为哈维氏弧菌( Vibrio harveyi )。毒性实验发现这两株菌都能引起斜带石斑鱼表皮溃烂症,其中201107-II毒性较强,被用于后续感染实验。通过肌肉注射、腹腔注射与创伤浸浴3种方式进行回归感染实验,得到3种方式对石斑鱼的半致死量LD50分别为4．3×104、2．9×105和5．4×106 cfu/g鱼体重。从人工感染的石斑鱼肾脏中再次分离到该菌,经生理、生化指标和16 S rRNA 基因鉴定,为同株菌。药敏实验结果表明,该菌株对红霉素、大观霉素、氯霉素等16种抗生素高度敏感,对呋喃唑酮、克拉霉素、环丙沙星等9种抗生素中度敏感,对苯唑西林、青霉素G、万古霉素等13种抗生素不敏感。本研究对石斑鱼病害防治与健康养殖具有一定的指导意义。
2011년7월,복건성진강모양식장양식적사대석반어( Epinephelus coioides )발병,병어체중3~10 g,증상위어체색변심,거식,리군완만유동,배기하방국부린편탈락、표피궤란,간장점상발홍。2011년7월종빈사병어간장、신장중분리도2주세균,분별명명위201107-I여201107-II,경생리、생화지표화16S rRNA 기인감정,저량주균위합유씨호균( Vibrio harveyi )。독성실험발현저량주균도능인기사대석반어표피궤란증,기중201107-II독성교강,피용우후속감염실험。통과기육주사、복강주사여창상침욕3충방식진행회귀감염실험,득도3충방식대석반어적반치사량LD50분별위4．3×104、2．9×105화5．4×106 cfu/g어체중。종인공감염적석반어신장중재차분리도해균,경생리、생화지표화16 S rRNA 기인감정,위동주균。약민실험결과표명,해균주대홍매소、대관매소、록매소등16충항생소고도민감,대부남서동、극랍매소、배병사성등9충항생소중도민감,대분서서림、청매소G、만고매소등13충항생소불민감。본연구대석반어병해방치여건강양식구유일정적지도의의。
Serious disease outbreak occurred in a groupers Epinephelus coioides culture farm in Jinjiang, Fu- <br> jian province in July 2011. The weight of diseased groupers was 3 ~10 g. The characteristics of diseased grouper were observed, displaying body colour darkening, feeding refused, swimming slowly, and redness of the liver and spleen spot. Two strains of bacteria were isolated from the liver and spleen of moribund groupers and designated as strain 201107-I and 201107-II in July 2011 . All the two isolates were identified as Vibrio harveyi by physiological and biochemical tests combining with 16S rRNA gene sequence analysis. Artificial in-jection demonstrated that strain 201107-I and 201107-II were both pathogenic to E. coioides. Strain 201107-II showed the stronger toxicity to E. coioides and was used to the subsequent analysis. Three experimental types of artificial infections were conducted, muscle injection, celiac injection and wound immersion, to con-firm the toxicity of strain 201107-II , and the half lethal dose ( LD50 ) to E. coioides was 4. 3 × 104 , 2. 9 × 105 and 5. 4 × 106 cfu/g fish weight, respectively. We also isolated a bacterial strain from this artificial infected E. coioides, and this strain was identified as the same strain as the 201107-II using traditional physiological and biochemical characterization along with molecular 16S rRNA gene analysis. The sensitivity tests of anti-bacterial agents indicated that the bacterial strain 201107 -II was sensitive to erythromycin, spectinomycin, chloramphenicol, and other 13 agents; middling sensitive to furazolidone, clarithromycin, and ciprofloxacin and other 6 agents;resistant to oxacillin, penicillin G, vancomycin and other 10 agents. This study will pro-vide necessary data about the sustainable and healthy development of E. coioides culture.