中外食品工业（下半月）
中外食品工業（下半月）
중외식품공업（하반월）
Sino-foreign Food Industry
2014年
8期
25-26
,共2页
花生油%黄曲霉毒素B1%ELISA%前处理方法%改进
花生油%黃麯黴毒素B1%ELISA%前處理方法%改進
화생유%황곡매독소B1%ELISA%전처리방법%개진
peanut oil%aflatoxin B1%ELISA%pretreatment method%improvement
目的：：对国标GB/T 5009.22-2003中第二法ELISA法测定花生油中黄曲霉毒素B1的样品前处理进行改良。方法：通过分别考察不同浓度的甲醇提取溶剂,分液漏斗萃取的不同振荡力度及频率,pH的调节对免疫反应的影响等。结果：提出了样品处理的最佳组合：使用60%甲醇提取液,分液漏斗萃取最优转速250rpm,振荡时间15min,样品提取物pH调节为6-8。新改进方法下样品加标回收率在80~110%之间。提高了ELISA法检测花生油黄曲霉毒素B1的效率和准确度。
目的：：對國標GB/T 5009.22-2003中第二法ELISA法測定花生油中黃麯黴毒素B1的樣品前處理進行改良。方法：通過分彆攷察不同濃度的甲醇提取溶劑,分液漏鬥萃取的不同振盪力度及頻率,pH的調節對免疫反應的影響等。結果：提齣瞭樣品處理的最佳組閤：使用60%甲醇提取液,分液漏鬥萃取最優轉速250rpm,振盪時間15min,樣品提取物pH調節為6-8。新改進方法下樣品加標迴收率在80~110%之間。提高瞭ELISA法檢測花生油黃麯黴毒素B1的效率和準確度。
목적：：대국표GB/T 5009.22-2003중제이법ELISA법측정화생유중황곡매독소B1적양품전처리진행개량。방법：통과분별고찰불동농도적갑순제취용제,분액루두췌취적불동진탕력도급빈솔,pH적조절대면역반응적영향등。결과：제출료양품처리적최가조합：사용60%갑순제취액,분액루두췌취최우전속250rpm,진탕시간15min,양품제취물pH조절위6-8。신개진방법하양품가표회수솔재80~110%지간。제고료ELISA법검측화생유황곡매독소B1적효솔화준학도。
objective:The ELISA detection method of Aflatoxin B1 in peanut oil should be optimized in national standard(GB/T 5009. 22--2003).Method:Respectively inspect different concentration of methanol extraction solvent,and extract different oscilation intensity and frequency with separating funnel,as well as study the effect of pH adjustment on the immune response.Result:Abstract the best combination of sample processing by using 60% methanol extract,the optional way of separating funnel extraction speech is 250rpm,the oscillation time is 15min,pH adjustment of sample extracts is 6—8.The sample standard addition recovery rate is between 80% and 110% with the new improved method.It helps improve the ELISA’s efficiency and accuracy of detecting Aflatoxin B1 in peanut oil.
