浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2014年
11期
1308-1310
,共3页
淋巴细胞亚群%CsA%造血干细胞移植
淋巴細胞亞群%CsA%造血榦細胞移植
림파세포아군%CsA%조혈간세포이식
lymphocyte subsets%cyclosporin A%hematopoietic stem cel transplantation
[目的]通过对急性髓细胞白血病移植患者服用环孢菌素A(cyclosporin A, CsA)前后外周血淋巴细胞亚群的检测，初步探讨CsA体内作用2h对淋巴细胞亚群的影响。[方法]采用流式细胞术检测43例急性髓细胞白血病移植后患者的空腹及服用CsA 2h的外周血T、B淋巴细胞，NK细胞的表达；同时应用化学发光法检测CsA的浓度。[结果]43例急性髓细胞白血病移植患者空腹CsA浓度为(125.43±65.94)ng·mL-1，服药2h浓度为(564.46±258.67) ng·mL-1；服药2h与空腹相比，CD3+细胞显著下调而NK细胞显著上升，但CD4+、CD8+、CD19+细胞及CD4+/CD8+却无明显变化。[结论]服用CsA 2h即能达到体内分布且影响CD3+细胞的数量，尚不能影响B细胞的数量。对于急性髓细胞白血病患者移植后服用CsA期间，有必要对CsA的浓度及淋巴细胞亚群进行监测以求达到合适的血药浓度，了解患者机体免疫状况，判断预后。
[目的]通過對急性髓細胞白血病移植患者服用環孢菌素A(cyclosporin A, CsA)前後外週血淋巴細胞亞群的檢測，初步探討CsA體內作用2h對淋巴細胞亞群的影響。[方法]採用流式細胞術檢測43例急性髓細胞白血病移植後患者的空腹及服用CsA 2h的外週血T、B淋巴細胞，NK細胞的錶達；同時應用化學髮光法檢測CsA的濃度。[結果]43例急性髓細胞白血病移植患者空腹CsA濃度為(125.43±65.94)ng·mL-1，服藥2h濃度為(564.46±258.67) ng·mL-1；服藥2h與空腹相比，CD3+細胞顯著下調而NK細胞顯著上升，但CD4+、CD8+、CD19+細胞及CD4+/CD8+卻無明顯變化。[結論]服用CsA 2h即能達到體內分佈且影響CD3+細胞的數量，尚不能影響B細胞的數量。對于急性髓細胞白血病患者移植後服用CsA期間，有必要對CsA的濃度及淋巴細胞亞群進行鑑測以求達到閤適的血藥濃度，瞭解患者機體免疫狀況，判斷預後。
[목적]통과대급성수세포백혈병이식환자복용배포균소A(cyclosporin A, CsA)전후외주혈림파세포아군적검측，초보탐토CsA체내작용2h대림파세포아군적영향。[방법]채용류식세포술검측43례급성수세포백혈병이식후환자적공복급복용CsA 2h적외주혈T、B림파세포，NK세포적표체；동시응용화학발광법검측CsA적농도。[결과]43례급성수세포백혈병이식환자공복CsA농도위(125.43±65.94)ng·mL-1，복약2h농도위(564.46±258.67) ng·mL-1；복약2h여공복상비，CD3+세포현저하조이NK세포현저상승，단CD4+、CD8+、CD19+세포급CD4+/CD8+각무명현변화。[결론]복용CsA 2h즉능체도체내분포차영향CD3+세포적수량，상불능영향B세포적수량。대우급성수세포백혈병환자이식후복용CsA기간，유필요대CsA적농도급림파세포아군진행감측이구체도합괄적혈약농도，료해환자궤체면역상황，판단예후。
Objective] By detecting the peripheral blood lymphocyte subsets of AML patients before and after taking cyclosporin A, preliminary discussion on making two hours effects of cyclosporin A on lymphocyte subsets in the body. [Methods] Fasting and taking cyclosporin A two hours of the expression of peripheral blood T, B lymphocytes and NK cel were detected by flow cytometry in 43 cases of patients with AML after transplantation. At the same time, application of chemiluminescence detection concentration of cyclosporin A. [Results] The results of cyclosporin A concentration: Fasting group is 125.43 ±65.94 ng·ml-1. Taking two hours group is 564.46 ±258.67 ng·ml-1; Taking two hours group compared with the fasting group, CD3+ cel is significantly reduced and NK cel is significantly increased, but CD4+, CD8+, CD19+cel and CD4+/CD8+ have no obvious change. [Conclusions] Taking cyclosporin A 2 hours can achieve distribution and affect the number of CD3+ cel in the body, but can not affect the B cel . To AML patients after transplantation, in order to achieve the appropriate concentration and keep abreast of the body's immune status of patients, it is necessary to monitor the concentration of cyclosporin A and lymphocyte subsets during their taking cyclosporin A, which helps us determine the prognosis.
