CAJ | 학술논문

目的：从欣脉胶囊中筛选出降血脂的主要活性部位，建立活性部位的HPLC指纹图谱，研究其与组方药材指纹图谱色谱峰的相关性。方法：利用溶剂萃取法对欣脉胶囊的不同极性部位进行分离制备，采用腹腔注射75%蛋黄乳液的方式快速建立小鼠高血脂症模型，筛选出欣脉胶囊的活性部位；建立欣脉胶囊活性部位及各药材的指纹图谱，以色谱峰保留时间为考察指标，对复方活性部位指纹图谱色谱峰归属进行分析。结果：乙酸乙酯部位和大蒜挥发油部位的降脂作用显著（P<0.01）；建立了欣脉胶囊活性部位的HPLC指纹图谱，标示出28个特征峰，对特征峰进行了归属分析，确定了27个特征峰的药材归属。结论：欣脉胶囊降血脂的主要活性部位为乙酸乙酯部位和大蒜挥发油部位，所建立的指纹图谱测定方法能较好地判别活性部位主要色谱峰与药材化学组分间的相关性，为欣脉胶囊的药效物质基础及质量标准提升提供参考。
목적：종흔맥효낭중사선출강혈지적주요활성부위，건립활성부위적HPLC지문도보，연구기여조방약재지문도보색보봉적상관성。방법：이용용제췌취법대흔맥효낭적불동겁성부위진행분리제비，채용복강주사75%단황유액적방식쾌속건립소서고혈지증모형，사선출흔맥효낭적활성부위；건립흔맥효낭활성부위급각약재적지문도보，이색보봉보류시간위고찰지표，대복방활성부위지문도보색보봉귀속진행분석。결과：을산을지부위화대산휘발유부위적강지작용현저（P<0.01）；건립료흔맥효낭활성부위적HPLC지문도보，표시출28개특정봉，대특정봉진행료귀속분석，학정료27개특정봉적약재귀속。결론：흔맥효낭강혈지적주요활성부위위을산을지부위화대산휘발유부위，소건립적지문도보측정방법능교호지판별활성부위주요색보봉여약재화학조분간적상관성，위흔맥효낭적약효물질기출급질량표준제승제공삼고。
This study was aimed to screen main active site to reduce blood lipid from Xin-Mai (XM) capsule and establish HPLC fingerprint of the site, in order to study the correlativity between active site and relevant fractions of its herbs. Solvent extraction was used to separate XM capsule into different polar fractions. Intraperitoneal injection of 75% egg-yolk emulsion was used to establish mice hyperlipidemia models. And the active site was screened. Chromatographic fingerprints of the site and relevant fractions of its herbs were configured by HPLC analysis. The <br> retention time of peaks was utilized as index to evaluate the correlativity. The results showed that lipid-lowering effect of ethyl acetate extract and garlic essential oil was significant (P<0.01). Fingerprint of the active site in XM capsule was established with 28 fingerprint peaks and the assignment results of 27 peaks were indicated. It was concluded that the active sites to reduce blood lipid of XM capsule were ethyl acetate extract and garlic essential oil. The established fingerprint method can effectively determine the correlativity between the active site and its relevant fractions, which contributed to pharmacodynamic material foundation and quality standard.