CAJ | 학술논문

目的：观察不同剂量D-氨基半乳糖（D-GalN）联合脂多糖（LPS）对大鼠急性肝衰竭模型的影响及凝血功能的变化，建立理想的急性肝衰竭大鼠模型。方法：SD大鼠随机分为正常组，D-GalN高、中、低剂量组，每组10只，除正常组外，其余各组注射不同剂量D-GalN联合LPS建立急性肝衰竭大鼠模型，观察大鼠的死亡率，检测大鼠0、12、24、48、72 h肝功能及凝血功能的水平；HE染色，观察肝脏病理的变化。结果：D-GalN高、中、低剂量组72 h内的死亡率分别为：60%、30%、10%；不同剂量组不同时间点血液中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（TBIL）、凝血酶原时间（PT）、国际标准比率（INR）、血浆纤维蛋白原（FIB）含量与正常组比较均有明显差异（P<0.05）；但在高、中、低剂量组间比较， ALT、AST无统计学意义，TBIL、PT、INR、FIB均有统计学差异（P<0.05）。结论：凝血功能在肝衰竭模型建立中为更加稳定的检测指标，通过肝功能、凝血功能、病理形态综合诊断，中剂量D-GalN联合LPS腹腔注射48 h后建立的急性肝衰竭大鼠模型与临床肝衰竭症状比较一致，中剂量是理想的造模剂量。
목적：관찰불동제량D-안기반유당（D-GalN）연합지다당（LPS）대대서급성간쇠갈모형적영향급응혈공능적변화，건립이상적급성간쇠갈대서모형。방법：SD대서수궤분위정상조，D-GalN고、중、저제량조，매조10지，제정상조외，기여각조주사불동제량D-GalN연합LPS건립급성간쇠갈대서모형，관찰대서적사망솔，검측대서0、12、24、48、72 h간공능급응혈공능적수평；HE염색，관찰간장병리적변화。결과：D-GalN고、중、저제량조72 h내적사망솔분별위：60%、30%、10%；불동제량조불동시간점혈액중병안산안기전이매（ALT）、천문동안산안기전이매（AST）、총담홍소（TBIL）、응혈매원시간（PT）、국제표준비솔（INR）、혈장섬유단백원（FIB）함량여정상조비교균유명현차이（P<0.05）；단재고、중、저제량조간비교， ALT、AST무통계학의의，TBIL、PT、INR、FIB균유통계학차이（P<0.05）。결론：응혈공능재간쇠갈모형건립중위경가은정적검측지표，통과간공능、응혈공능、병리형태종합진단，중제량D-GalN연합LPS복강주사48 h후건립적급성간쇠갈대서모형여림상간쇠갈증상비교일치，중제량시이상적조모제량。
This study was aimed to observe effects of different doses of D-galactosamine (D-GalN) plus lipopoly-saccharide (LPS) and blood coagulation changes among rat model of acute liver failure, in order to establish an ideal model of acute liver failure in rats. SD rats were randomly divided into the control group, D-GalN high, medium and low dose groups, with 10 rats in each group. Except the normal group, rats in other groups were injected with D-GalN plus LPS at different doses to induce acute liver failure. The mortality of rats was observed. The liver function and blood coagulation were detected from rat serum at 0 h, 12 h, 24 h, 48 h, and 72 h. HE stain was used in the observa-tion of changes on liver pathological changes. The results showed that the mortality of D-GalN high, medium and low dose groups within 72 h were 60%, 30%, 10%, respectively. There were significant differences on the serum content level of ALT, AST, TBIL, PT, INR, FIB from different dose groups at different time points and the normal group (P<0.05). However, the comparison among D-GalN high, medium and low dose groups showed no statistical difference on ALT and AST; while there were statistical differences on TBIL, PT, INR and FIB (P < 0.05). It was concluded that coagulation index was more stable in the liver failure model. Through observation on the liver function, blood coagulation and pathological morphology, the model of acute liver failure induced with medium dose of D-GalN plus LPS in SD rats at 48 h was more similar to the clinical symptom of acute liver failure. Therefore, the medium dose was the ideal model inducing dose.