CAJ | 학술논문

目的：建立一种同时测定冬虫夏草中尿苷、肌苷、鸟苷、腺苷和虫草素5种核苷类成分含量的高效液相色谱法。方法：ZORBAX SB-Aq（150 mm×4.6 mm，5μm）色谱柱，0.1%甲酸水溶液-乙腈梯度洗脱，检测波长260 nm，柱温30℃，流速0.8 mL·min-1。结果：对6批冬虫夏草样本子座、虫体和全草的核苷类成分进行了分析，结果发现冬虫夏草子座核苷类成分含量高于虫体和全草。结论：该方法重复性好，准确性高，可用于冬草夏草中核苷类成分的含量测定。
목적：건립일충동시측정동충하초중뇨감、기감、조감、선감화충초소5충핵감류성분함량적고효액상색보법。방법：ZORBAX SB-Aq（150 mm×4.6 mm，5μm）색보주，0.1%갑산수용액-을정제도세탈，검측파장260 nm，주온30℃，류속0.8 mL·min-1。결과：대6비동충하초양본자좌、충체화전초적핵감류성분진행료분석，결과발현동충하초자좌핵감류성분함량고우충체화전초。결론：해방법중복성호，준학성고，가용우동초하초중핵감류성분적함량측정。
A HPLC method for simultaneous determination of uridine, inosine, guanosine, adenosine and cordycepine <br> in Cordyceps sinensis was developed. The sample solution was prepared with 0.5% phosphoric acid solution by ultrasonic extraction. The separation was performed on a ZORBAX SB-AQ (150 mmí4.6 mm, 5 μm)column with gradient elution by 0.1% formic acid solution and acetonitrile, with column temperature 30℃, at a flow rate of 0.8 mL/min, and detected at wavelength of 260 nm. The result of method validation showed that the developed method had high accuracy and good repeatability. This method has been successfully applied for analysis of 5 kinds of nucleosides in different parts of C. sinensis. The results indicated that content of nucleosides in stroma is higher than that in insect body and whole C. sinensis.