CAJ | 학술논문

目的：观察针刺风池穴对RAMP1和5－HT1B受体的作用，探讨风池穴对偏头痛CGRP和5－HT信号的可能影响。方法：采用免疫组化的方法，观察了针刺风池穴对偏头痛模型大鼠脑膜中动脉RAMP1和5－HT1B受体的影响。结果：电刺激大鼠硬脑膜可引起脑膜中动脉RAMP1增多、5－TH1B受体减少，无论是在造模前针刺风池穴还是在造模后针刺风池穴，RAMP1表达显著减少，而对5－TH1B受体并没有显著影响。结论：在偏头痛发作期，存在着CGRP作用的激活和5－TH作用的抑制，针刺风池穴显然能影响偏头痛发作过程中的血管反应，而其主要作用可能是通过CGRP途径实现的。
목적：관찰침자풍지혈대RAMP1화5－HT1B수체적작용，탐토풍지혈대편두통CGRP화5－HT신호적가능영향。방법：채용면역조화적방법，관찰료침자풍지혈대편두통모형대서뇌막중동맥RAMP1화5－HT1B수체적영향。결과：전자격대서경뇌막가인기뇌막중동맥RAMP1증다、5－TH1B수체감소，무론시재조모전침자풍지혈환시재조모후침자풍지혈，RAMP1표체현저감소，이대5－TH1B수체병몰유현저영향。결론：재편두통발작기，존재착CGRP작용적격활화5－TH작용적억제，침자풍지혈현연능영향편두통발작과정중적혈관반응，이기주요작용가능시통과CGRP도경실현적。
Objective:To assess the possible effects of acupuncture at GB 20 ( Fengchi) on the CGRP and 5-HT signal of hemicrania based on the influence of inserting GB 20 ( Fengchi) on RAMP1 and 5-HT1B recep-tors.Methods:We used immunohistochemical staining to observe the influence of inserting GB 20 (Fengchi) on RAMP1 and 5-HT1B receptors.Results:Electrical stimulation of rats’ dura could cause the increase of RAMP1 and the decrease of 5-TH1B receptors of middle meningeal arteries.The expression of RAMP1 was significantly <br> reduced when acupuncture at GB 20 ( Fengchi) both before and after the modeling, while there was no signifi-cant changes of the expression of 5-TH1B .Conclusion:On acute attack of hemicrania, there is activation of CGRP and inhabitation of 5-TH.Acupuncture at GB 20 (Fengchi) can influence the blood response during the attack of hemicrania, and probably act mainly through CGRP path.