CAJ | 학술논문

目的：探讨蜂毒多肽对人膀胱癌T24细胞浸润能力的影响。方法将不同浓度(0、0.1、1.0、10.0、100.0 mg/ml)蜂毒多肽作用于人膀胱癌T24细胞(对应对照组及不同浓度蜂毒多肽组),分别采用四甲基偶氮唑盐( MTT)法、Transwell小室法及划痕法检测人膀胱癌T24细胞的黏附、浸润及迁移能力。结果不同浓度蜂毒多肽组黏附率均较对照组降低,差异均有统计学意义(P<0．05)；且随着蜂毒多肽浓度升高黏附率逐渐下降,不同浓度蜂毒多肽组间两两比较差异亦均有统计学意义(P<0．05)。培养24 h时不同浓度蜂毒多肽组透过膜的细胞数均较对照组减少,差异均有统计学意义(P<0．05)；且随着蜂毒多肽浓度升高透过膜的细胞数逐渐减少,不同浓度蜂毒多肽组间两两比较差异亦均有统计学意义(P<0．05)。划痕后12、24 h,不同浓度蜂毒多肽组细胞未覆盖面积均较对照组同一时间增加,差异均有统计学意义(P<0．05)；且随着蜂毒多肽浓度升高细胞未覆盖面积逐渐增加,不同浓度蜂毒多肽组同一时间两两比较差异亦均有统计学意义( P<0．05)。结论蜂毒多肽可抑制人膀胱癌T24细胞的黏附、浸润和迁移能力,且随着蜂毒多肽浓度的升高,其抑制作用增强,呈剂量依赖性。
목적：탐토봉독다태대인방광암T24세포침윤능력적영향。방법장불동농도(0、0.1、1.0、10.0、100.0 mg/ml)봉독다태작용우인방광암T24세포(대응대조조급불동농도봉독다태조),분별채용사갑기우담서염( MTT)법、Transwell소실법급화흔법검측인방광암T24세포적점부、침윤급천이능력。결과불동농도봉독다태조점부솔균교대조조강저,차이균유통계학의의(P<0．05)；차수착봉독다태농도승고점부솔축점하강,불동농도봉독다태조간량량비교차이역균유통계학의의(P<0．05)。배양24 h시불동농도봉독다태조투과막적세포수균교대조조감소,차이균유통계학의의(P<0．05)；차수착봉독다태농도승고투과막적세포수축점감소,불동농도봉독다태조간량량비교차이역균유통계학의의(P<0．05)。화흔후12、24 h,불동농도봉독다태조세포미복개면적균교대조조동일시간증가,차이균유통계학의의(P<0．05)；차수착봉독다태농도승고세포미복개면적축점증가,불동농도봉독다태조동일시간량량비교차이역균유통계학의의( P<0．05)。결론봉독다태가억제인방광암T24세포적점부、침윤화천이능력,차수착봉독다태농도적승고,기억제작용증강,정제량의뢰성。
Objective To investigate the effect of venom peptides on the invasion of human bladder carcinoma cell line T24. Methods Various concentrations of venom peptides (0, 0. 1, 1. 0, 10. 0, 100. 0 mg/ml) were acted on to human bladder cancer cell line T24 ( control group and venom peptides group in different concentrations) in order to examine the ad-hesion,infiltration and migration ability of human bladder cancer cell line T24 by MTT assay, Transwell assay and scratch test respectively. Results Adhesion rate of various concentrations of venom peptides group was significantly lower than that of control group (P<0. 05);while the percentage of venom peptides increased, adhesion rate decreased progressively, and there was significant difference between the two groups in various concentrations of venom peptides groups (P<0. 05). Cell popula-tion penetrating the membrane in the 24 hour culture of various concentrations of venom peptides group was significantly de-creased, compared with that in control group ( P<0. 05 ); Cell population penetrating the membrane decreased progressively in the wake of the concentration of venom peptides increasing, and there was significant difference between the two groups in various concentrations of venom peptides group (P<0. 05). 12 and 24 hours after scratching, cell non-coverage area of vari-ous concentrations of venom peptides group increased, compared with that of control group at the same time, and there was significant difference between the two groups in various concentrations of venom peptides group (P<0. 05);Cell non-coverage area increased progressively along with the concentration of venom peptides increasing, and there was significant difference be-tween the two groups at the same time in various concentrations of venom peptides group ( P<0. 05 ) . Conclusion Venom peptide possesses an inhibitory effect against the adhesion,infiltration and migration ability of human bladder cancer cell line T24, which increases along with the increasing of concentrations of venom peptides in a dose-dependent manner.