CAJ | 학술논문

为使合成聚乙二醇化纳米氧化石墨烯（ NGO ）在生理条件下稳定保存，首先采用改进的Hummers 法合成NGO，再通过酰胺反应在NGO连接四臂聚乙二醇，通过测定FT-IR光谱、紫外分光光谱、粒度与粒度分布、SEM与TEM对产物进行表征。产物中含有羟基、羧基、羰基等含氧官能团，紫外最大吸收峰为225 nm，表明NGO与GO-PEG的成功合成。 NGO和GO-PEG的平均粒径分别为184．08 nm和96．72 nm，PDI分别为0．317和0．365。 SEM， TEM数据揭示了产物的片状形貌，GO-PEG在PBS和细胞培养基（ DMEM）中静置60 d，未出现聚集和沉降现象，表明GO-PEG具有良好的体外稳定性。文中成功制备了NGO和聚乙二醇化的NGO，并且聚乙二醇化后的NGO在PBS和DMEM中具有良好的体外稳定性，为其在生物医药领域的应用奠定了基础。
위사합성취을이순화납미양화석묵희（ NGO ）재생리조건하은정보존，수선채용개진적Hummers 법합성NGO，재통과선알반응재NGO련접사비취을이순，통과측정FT-IR광보、자외분광광보、립도여립도분포、SEM여TEM대산물진행표정。산물중함유간기、최기、탄기등함양관능단，자외최대흡수봉위225 nm，표명NGO여GO-PEG적성공합성。 NGO화GO-PEG적평균립경분별위184．08 nm화96．72 nm，PDI분별위0．317화0．365。 SEM， TEM수거게시료산물적편상형모，GO-PEG재PBS화세포배양기（ DMEM）중정치60 d，미출현취집화침강현상，표명GO-PEG구유량호적체외은정성。문중성공제비료NGO화취을이순화적NGO，병차취을이순화후적NGO재PBS화DMEM중구유량호적체외은정성，위기재생물의약영역적응용전정료기출。
The nanoscale graphene oxide ( NGO ) was synthesized and pegylated in order to impart stability in physiological environment . The graphene oxide was prepared by modified Hummers method , and then functionalized with 4-arm polyethylene glycol via amide reaction .The structure and morphology of NGO and NGO-PEG were characterized by FT-IR, UV-Vis, SEM and TEM, respectively.The result shows that the NGO prepared has a morphology of lamellar layer which contains the oxygen-based functional groups such as —OH,—COOH and C O.The maximum UV absorption wavelength of NGO and NGO-PEG is in 225 nm, which presents the successful synthesis of NGO and NGO-PEG.The average particle sizes of NGO and NGO-PEG are 184.08 nm and 96.72 nm, respectively, with PDI of 0.317 and 0.365, respectively.NGO-PEG exhibits good stability in PBS and DMEM solution without any agglomeration during 60 d.It concludes that the nanoscale graphene oxide and pegylated NGO are prepared successfully and NGO-PEG is stable in PBS and DMEM solution , which has widespread prospect for biomedical application .