CAJ | 학술논문

目的：为筛选云南高海拔牧场牦牛高效力的寄生线虫生防真菌菌株。方法：对云南大理花甸坝牧场，中甸打日坝牧场及白马雪山牧场采集的少孢节丛孢（A. oligospora）、弯孢节丛孢（A. musiformis）、椭圆隔指孢（D. ellipsospora）和长孢隔指孢（D. leptospora）采用盐酸胃蛋白酶处理筛选耐受菌株，并进一步比较了耐受菌株的捕食活力。结果：12株捕食线虫真菌中，有6株通过盐酸胃蛋白酶处理，通过率为50%，其中大理花甸坝和中甸打日坝的A. oligospora及白马雪山牧场的D. ellipsospora显示出较强的捕食活力。结论：本研究初步筛选出3株生防高效菌株，为牦牛及其它动物线虫病的生物防治积累了捕食线虫真菌菌种资源。
목적：위사선운남고해발목장모우고효력적기생선충생방진균균주。방법：대운남대리화전패목장，중전타일패목장급백마설산목장채집적소포절총포（A. oligospora）、만포절총포（A. musiformis）、타원격지포（D. ellipsospora）화장포격지포（D. leptospora）채용염산위단백매처리사선내수균주，병진일보비교료내수균주적포식활력。결과：12주포식선충진균중，유6주통과염산위단백매처리，통과솔위50%，기중대리화전패화중전타일패적A. oligospora급백마설산목장적D. ellipsospora현시출교강적포식활력。결론：본연구초보사선출3주생방고효균주，위모우급기타동물선충병적생물방치적루료포식선충진균균충자원。
Objective: To screen high efficiency biocontrol strains of nematode-trapping fungi isolated from yak dung in plateau pastures of Yunnan Province. Methods:Twelve nematode-trapping fungi of A. oligospora, A. musiformis, D. ellipsospora, D. leptospora isolated from Dali Huadianba pasture, Zhongdian Dariba pasture, Baima Snow Mountain pasture were tested in pepsin-hydrochloric acid and the nematode-trapping activity was determined subsequently. Results:Half of isolates survived in pepsin-hydrochloric acid treatment and three isolates A. oligospora from Huadianba pasture and Dariba pasture and D. ellipsospora from Baima Snow Mountain pasture displayed high nematode-trapping activity under laboratory conditions. Conclusion: Three high efficiency biocontrol nematode-trapping fungi were obtained and this research accumulated biocontrol strain resources in the biological control of nematode disease of yak and other animals.