CAJ | 학술논문

目的：观察电针对大脑中动脉缺血再灌注模型大鼠Nrf2蛋白表达的影响。方法根据数字随机表将15只 SD健康雄性大鼠随机分为假手术组、模型组和电针组，每组各5只。用改良线栓法制备大鼠局灶性缺血再灌注模型，再灌注的同时给予电针组大鼠“百会”、“大椎”两穴30 min电针（深度均为10 mm，连续波，频率3 Hz，电流强度1～3 mA），免疫组织化学法检测Nrf2蛋白的表达，采用图象分析系统测定视野中Nrf2蛋白阳性细胞的平均灰度、平均光密度。结果与假手术组相比，模型组大脑皮层锥体细胞层表达Nrf2蛋白的阳性细胞数稍有增多，但尚未有统计学意义，着色亦无明显改变；而电针组可见到大量的Nrf2蛋白阳性细胞数，与模型组、假手术组相比，有显著统计学差异（P＜0.01，P＜0.05），平均灰度显著降低，平均光密度显著升高（P均＜0.01）。结论电针可通过增加Nrf2蛋白的表达来发挥其调节抗氧化酶水平以达到保护机体，免受自由基损伤的目的。
목적：관찰전침대대뇌중동맥결혈재관주모형대서Nrf2단백표체적영향。방법근거수자수궤표장15지 SD건강웅성대서수궤분위가수술조、모형조화전침조，매조각5지。용개량선전법제비대서국조성결혈재관주모형，재관주적동시급여전침조대서“백회”、“대추”량혈30 min전침（심도균위10 mm，련속파，빈솔3 Hz，전류강도1～3 mA），면역조직화학법검측Nrf2단백적표체，채용도상분석계통측정시야중Nrf2단백양성세포적평균회도、평균광밀도。결과여가수술조상비，모형조대뇌피층추체세포층표체Nrf2단백적양성세포수초유증다，단상미유통계학의의，착색역무명현개변；이전침조가견도대량적Nrf2단백양성세포수，여모형조、가수술조상비，유현저통계학차이（P＜0.01，P＜0.05），평균회도현저강저，평균광밀도현저승고（P균＜0.01）。결론전침가통과증가Nrf2단백적표체래발휘기조절항양화매수평이체도보호궤체，면수자유기손상적목적。
Objective To investigate the effect of electro-acupuncture(EA) on the expression of Nrf2 protein in cerebral cortex of acerebral ischemia reperfusion rat model.Methods 15 healthy SD male rats were randomly divided into control, model and EA groups, 5 ratsin each group.A model of reversible middle cerebral artery occlusion in rat was prepared via the modified thread occlusion method.In EAgroup, 30 min EA stimulation was applied to both 'Baihui'and 'Dazhui'points in each rat (10 mm EA penetration depth, continuous wavewith a frequency of 3 Hz and an current intensity of 1 ~3 mA), while the ischemia reperfusion was operating.The expression of Nrf2 proteinwas assessed by immune-histochemistry method.The averaged level and optical density of Nrf2 protein positive neurons were examined by imaginganalysis system.Results Compared to that of control group, the number of positive neurons with Nrf2 protein expression in the cerebralcortex pyramidal cell layer slightly was increased in model group, but there was no statistical significance.Compared to that of controland model groups, the number of positive neurons with Nrf2 protein expression in the cerebral cortex pyramidal cell layer was significantly increasedin EA group(P<0.01, P<0.05),while the averaged gray level was significantly decreased (P<0.01)and the averaged optical densitywas significantly increased(P<0.01).Conclusions Nrf2 protein expression could be significantly enhanced with EA stimulation, whichis helpful for regulating the anti-oxidant enzyme level to achieve the goal of protecting the body from free radical injury.