CAJ | 학술논문

目的：探讨低剂量辐射（LDR）联合阿霉素（ADM）对荷乳腺癌裸鼠肾脏组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、丙二醛（MDA）的影响。方法36只荷乳腺癌裸鼠，随机分为6个试验组（每组6只）：正常对照组，荷瘤假照组（0 mGy），LDR组（75 mGy）， ADM组，LDR-6 h-ADM组（LDR后6 h给予ADM），LDR-12 h-ADM组（LDR后12 h给予ADM）。各试验组以不同方式处理后4 d，荷乳腺癌裸鼠全部处死，取新鲜左侧肾脏组织匀浆上清液，检测SOD、GSH-PX活力、MDA 含量。结果与正常对照组相比，荷瘤假照组 SOD 和 GSH-PX 值显著降低， MDA值显著增高（P＜0.05）；与荷瘤假照组相比，ADM组SOD、GSH-PX值均明显下降，MDA值明显增高，LDR组 SOD、GSH-PX值均显著增高，MDA值显著降低（P＜0.05）；与ADM组相比，LDR＋ADM组的SOD、GSH-PX值明显增高，MDA明显降低（P＜0.05）。结论 LDR 可能增强荷乳腺癌裸鼠肾脏组织抗氧化酶活性，减少脂质过氧化产物生成，抑制氧化应激反应，从而减轻ADM对肾脏组织的损伤。
목적：탐토저제량복사（LDR）연합아매소（ADM）대하유선암라서신장조직초양화물기화매（SOD）、곡광감태과양화물매（GSH-PX）、병이철（MDA）적영향。방법36지하유선암라서，수궤분위6개시험조（매조6지）：정상대조조，하류가조조（0 mGy），LDR조（75 mGy）， ADM조，LDR-6 h-ADM조（LDR후6 h급여ADM），LDR-12 h-ADM조（LDR후12 h급여ADM）。각시험조이불동방식처리후4 d，하유선암라서전부처사，취신선좌측신장조직균장상청액，검측SOD、GSH-PX활력、MDA 함량。결과여정상대조조상비，하류가조조 SOD 화 GSH-PX 치현저강저， MDA치현저증고（P＜0.05）；여하류가조조상비，ADM조SOD、GSH-PX치균명현하강，MDA치명현증고，LDR조 SOD、GSH-PX치균현저증고，MDA치현저강저（P＜0.05）；여ADM조상비，LDR＋ADM조적SOD、GSH-PX치명현증고，MDA명현강저（P＜0.05）。결론 LDR 가능증강하유선암라서신장조직항양화매활성，감소지질과양화산물생성，억제양화응격반응，종이감경ADM대신장조직적손상。
Objective To study the effects of low dose radiation ( LDR ) combined with adriamycin on superoxide dismutase ( SOD) , glutathione peroxidase ( GSH-PX) , malondialdehyde ( MDA) in kidney tissue of breast cancer-bearing nude mice.Methods Thir-ty-six breast cancer-bearing nude mice were divided into normal control, sham-irradiated(0 mGy), LDR(75 mGy), ADM and LDR-6 h-ADM, LDR-12 h-ADM groups.Take fresh kidney tissue homogenate was obtained to assay the vitality of SOD, GSH-PX and MDA con-tentResults Compared to those of normal control group, the value of SOD and GSH-PX were significantly decreased and the value of MDA was significantly increased in sham-irradiated group(P<0.05).Compared with those of sham-irradiated group, the value of SOD, GSH-PX in ADM group were decreased, while those in LDR group were increased;the value of MDA in ADM group was increased while that in LDR group was decreased(P<0.05).Compared to those of ADM group, the value of SOD and GSH-PX were increased and the value of MDA were decreased in LDR+ADM group(P<0.05).Conclusions LDR could enhance the antioxidant enzyme activity in kidney tissue of breast tumor-bearing mouse, and reduce lipid peroxidation formation producing, thereby inhibiting oxidative stress reaction to reduce doxorubicin-in-duced kidney injury.