CAJ | 학술논문

目的：探讨冰冻切片制作过程中的常见问题及解决方法。方法针对8126例甲状腺、子宫、乳腺、脂肪、脑、卵巢、淋巴结、肺、皮肤、肠息肉等不同组织在冰冻切片制作过程中出现的组织脱片、组织收缩、制片困难及冰晶过多和染色时间长、染色效果不佳等问题进行改进。结果通过对取材组织的预处理，包埋剂用量的把握，冷冻时间的控制以及酒精灯和胶片的配合使用，圆满地解决了冰冻切片制作过程中的冰晶过多、、组织收缩、组织脱片等问题。结论冰冻切片中对组织取材、包埋、冷冻及染色的改进，有助于及时准确地制作出优质的冰冻切片，为明确诊断提供了良好的技术支持和条件保障。
목적：탐토빙동절편제작과정중적상견문제급해결방법。방법침대8126례갑상선、자궁、유선、지방、뇌、란소、림파결、폐、피부、장식육등불동조직재빙동절편제작과정중출현적조직탈편、조직수축、제편곤난급빙정과다화염색시간장、염색효과불가등문제진행개진。결과통과대취재조직적예처리，포매제용량적파악，냉동시간적공제이급주정등화효편적배합사용，원만지해결료빙동절편제작과정중적빙정과다、、조직수축、조직탈편등문제。결론빙동절편중대조직취재、포매、냉동급염색적개진，유조우급시준학지제작출우질적빙동절편，위명학진단제공료량호적기술지지화조건보장。
Objective To explore the common problems and solutions of cryostat technique. Methods In this retrospective study,a total of 8 126 surgical fresh specimens of thyroid,uterus,breast,fat,brain,ovary,lymph nodes,lung,skin,etc. were sliced and different problems had been met,for example,tissue detachment,tissue shrinkage in different sections,creation of ice crystals,etc. The corresponding solutions had been solved. Results The improvement of surgical cutup,embedding media and freezing duration and the joint application of alcohol burner and anti-escape of slides overcoming the encountered problems. Conclusion The modified method can quickly and accurately provide good quality of sections for pathologists.