CAJ | 학술논문

目的：研究鞘内注射TRESK基因重组腺病毒（ pAd/CMV/V5-DEST-TRESK）对坐骨神经分支选择性损伤（SNI）大鼠痛觉过敏的影响。方法雄性SD大鼠36只，随机分为6组（n＝6）：Ⅰ组为空白对照组；Ⅱ组为假手术组；Ⅲ组为SNI模型组；Ⅳ组大鼠制备SNI模型后鞘内单次注射生理盐水25μl；Ⅴ组和Ⅵ组大鼠制备SNI模型后，鞘内分别单次注射滴度为1.31×109 TU/ml的阴性腺病毒和pAd/CMV/V5-DEST-TRESK。各组大鼠于术前1 d及术后1 d、3 d、5 d、7 d、14 d分别测定机械痛阈和热痛阈；术后14 d检测L4，5术侧背根神经节（ DRG）的TRESK蛋白表达。结果Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组术后1d、3d、5d、7d、14d的机械痛阈明显低于Ⅰ组（P ﹤0.05），Ⅲ组、Ⅳ组、Ⅴ组5d、7d、14d的机械痛阈明显低于Ⅵ组（ P ﹤0.05）；与术前比较，Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组术后1~14 d的机械痛阈明显降低（ P ﹤0.05）。Ⅲ组、Ⅳ组、Ⅴ组、Ⅵ组L4，5术侧DRG的TRESK蛋白表达明显低于Ⅰ组（ P ﹤0.05），Ⅵ组L4，5术侧DRG的TRESK蛋白表达明显高于Ⅲ组、Ⅳ组、Ⅴ组（ P﹤0.05）。结论鞘内注射pAd/CMV/V5-DEST-TRESK可上调SNI大鼠DRG的TRESK蛋白表达，减轻SNI大鼠的痛觉过敏。
목적：연구초내주사TRESK기인중조선병독（ pAd/CMV/V5-DEST-TRESK）대좌골신경분지선택성손상（SNI）대서통각과민적영향。방법웅성SD대서36지，수궤분위6조（n＝6）：Ⅰ조위공백대조조；Ⅱ조위가수술조；Ⅲ조위SNI모형조；Ⅳ조대서제비SNI모형후초내단차주사생리염수25μl；Ⅴ조화Ⅵ조대서제비SNI모형후，초내분별단차주사적도위1.31×109 TU/ml적음성선병독화pAd/CMV/V5-DEST-TRESK。각조대서우술전1 d급술후1 d、3 d、5 d、7 d、14 d분별측정궤계통역화열통역；술후14 d검측L4，5술측배근신경절（ DRG）적TRESK단백표체。결과Ⅲ조、Ⅳ조、Ⅴ조、Ⅵ조술후1d、3d、5d、7d、14d적궤계통역명현저우Ⅰ조（P ﹤0.05），Ⅲ조、Ⅳ조、Ⅴ조5d、7d、14d적궤계통역명현저우Ⅵ조（ P ﹤0.05）；여술전비교，Ⅲ조、Ⅳ조、Ⅴ조、Ⅵ조술후1~14 d적궤계통역명현강저（ P ﹤0.05）。Ⅲ조、Ⅳ조、Ⅴ조、Ⅵ조L4，5술측DRG적TRESK단백표체명현저우Ⅰ조（ P ﹤0.05），Ⅵ조L4，5술측DRG적TRESK단백표체명현고우Ⅲ조、Ⅳ조、Ⅴ조（ P﹤0.05）。결론초내주사pAd/CMV/V5-DEST-TRESK가상조SNI대서DRG적TRESK단백표체，감경SNI대서적통각과민。
Objective To investigate the effects of recombinant adenovirus vector containing TRESK gene( pAD/CMV/V5 -DEST-TRESK)by intrathecal injection on hyperalgesia of sciatic nerve branch selective injury( SNI)rats. Methods Thirty six SD male rats were ran_domly divided into six groups,six rats in each groups:control group( Group I);sham operation group( GroupⅡ);SNI group( GroupⅢ). SNI rats were given respectively single intrathecal injected 25μl physiological saline. The negative adenovirus and pAD/CMV/V5-DEST-TRESK in Group Ⅳ,Group Ⅴ and GroupⅥ,the titer of adenovirus was 1. 31 × 109 TU/ml. Rats in each groups were determined by mechanical threshold (MWT)and heat pain threshold respectively(TWL)at 1d before operation and 1,3,5,7,14 d after operation. The protein of TRESK in the side of L4,5 DRG were determined at 14 d after operation. Results The MWT were significantly lower in GroupⅢ,Ⅳ,ⅤandⅥthan in Group I at 1d before operation and 1,3,5,7,14 d after operation( P ﹤0. 05). The MWT were significantly lower in GroupⅢ,ⅣandⅤthan in GroupⅥat 5,7,14 d after operation( P ﹤0. 05). The MWT in GroupⅢ,Ⅳ,ⅤandⅥwere significantly lower at 1,3,5,7,14 day after operation than at 1 day before operation( P ﹤0. 05). The protein of TRESK in DRG in GroupⅢ,Ⅳ,ⅤandⅥwere significantly lower than in Group I ( P ﹤0. 05). The protein of TRESK in DRG in GroupⅥwere significantly higher than in GroupⅢ,ⅣandⅤ( P ﹤0. 05). Conclusion In_trathecal injection with the recombinant adenovirus vector containing TRESK gene( pAD/CMV/V5-DEST-TRESK )can up-regulate the pro_tein of TRESK in DRG and alleviate the hyperalgesia of SNI rats.