CAJ | 학술논문

目的：探讨血管紧张素1-7（Ang 1-7）干预雨蛙素（caerulein，CAE）刺激的胰腺腺泡细胞AR42J后，p38 MAPK/NF-κB信号蛋白的表达变化。方法将AR42J细胞随机分为3组：对照组、雨蛙素组（10 nmol/L，CAE组）、Ang 1-7组（10-7 mol/L、10-6 mol/L、10-5 mol/L Ang 1-7＋10 nmol/L雨蛙素）。对照组为正常生长的AR42J细胞，CAE组用10 nmol/L的雨蛙素刺激AR42J于2 h、6 h、12 h、24 h及48 h收集细胞，Ang 1-7组为不同浓度Ang 1-7作用30 min后再用雨蛙素刺激24 h收集细胞，Western blot技术检测各组细胞中ACE2、Mas受体、p38 MAPK、p-p38 MAPK及NF-κB的蛋白表达。结果 AR42J细胞中可见ACE2-Ang 1-7-Mas受体轴表达；与对照组相比，CAE组ACE2和Mas受体的表达呈先增多后减少的趋势，均于24 h达高峰（ P ＜0.05）；p38 MAPK、p-p38 MAPK及NF-κB的表达亦是24 h达峰值（ P ＜0.05）；Ang 1-7（10-7 mol/L、10-6 mol/L及10-5 mol/L）上调Mas受体的表达，下调p38 MAPK、p-p38 MAPK、NF-κB蛋白的表达，Ang 1-7的浓度为10-5 mol/L时，后三者的表达水平与CAE组比较，差异均具有统计学意义（ P ＜0.05）。结论雨蛙素刺激的AR42J细胞中，Ang 1-7可通过Mas受体抑制p38MAPK/NF-κB信号通路的活性。
목적：탐토혈관긴장소1-7（Ang 1-7）간예우와소（caerulein，CAE）자격적이선선포세포AR42J후，p38 MAPK/NF-κB신호단백적표체변화。방법장AR42J세포수궤분위3조：대조조、우와소조（10 nmol/L，CAE조）、Ang 1-7조（10-7 mol/L、10-6 mol/L、10-5 mol/L Ang 1-7＋10 nmol/L우와소）。대조조위정상생장적AR42J세포，CAE조용10 nmol/L적우와소자격AR42J우2 h、6 h、12 h、24 h급48 h수집세포，Ang 1-7조위불동농도Ang 1-7작용30 min후재용우와소자격24 h수집세포，Western blot기술검측각조세포중ACE2、Mas수체、p38 MAPK、p-p38 MAPK급NF-κB적단백표체。결과 AR42J세포중가견ACE2-Ang 1-7-Mas수체축표체；여대조조상비，CAE조ACE2화Mas수체적표체정선증다후감소적추세，균우24 h체고봉（ P ＜0.05）；p38 MAPK、p-p38 MAPK급NF-κB적표체역시24 h체봉치（ P ＜0.05）；Ang 1-7（10-7 mol/L、10-6 mol/L급10-5 mol/L）상조Mas수체적표체，하조p38 MAPK、p-p38 MAPK、NF-κB단백적표체，Ang 1-7적농도위10-5 mol/L시，후삼자적표체수평여CAE조비교，차이균구유통계학의의（ P ＜0.05）。결론우와소자격적AR42J세포중，Ang 1-7가통과Mas수체억제p38MAPK/NF-κB신호통로적활성。
Objective To study the effect of Angiotensin 1-7(Ang 1-7)on the p38 MAPK/NF-κB signaling pathway in caerulein-stimulated pancreatic acinar AR42J cell. Methods AR42J cells were randomly divided into 3 groups,including control group,caerulein group (10 nmol/L,CAE)and Ang 1-7 group(10 -7、10 -6、10 -5 mol/L Ang 1-7 + 10 nmol/L CAE). The control group was AR42J cell growing normally. AR42J cells were stimulated with 10 nmol/L caerulein for varying time periods and collected at 2 h,6 h,12 h,24 h and 48 h post stim-ulation. Before the stimulation of caerulein for 24 h,the Ang 1-7 group cells were mock pretreated or pretreated with different concentrations of Ang 1-7 for 30 min and 24 h post the treatments of caerulein,each group ACE2,Mas receptor,p38MAPK,p-p38MAPK,and NF-κB ex-pression levels were evaluated by Western Blotting. Results ACE2-Ang 1-7-Mas axis existed in AR42J cell. Compared with control group, the expression of ACE2 and Mas receptor of CAE group increased and then decreased ,both peaked at 24 h( P <0. 05). The p38 MAPK,p-p38 MAPK and NF-κB were also peaked at 24 h( P <0. 05). Ang 1-7 group(10 -7 mol/L,10 -6 mol/L and 10 -5 mol/L)up-regulated the expression of Mas receptor,down-regulated p38 MAPK,p-p38 MAPK,NF-κB expression. When the concentration of Ang 1 -7 was 10 -5 mol/L,the latter three protein levels were statistically significant( P <0. 05)compared with CAE group. Conclusion In the caerulein-stimulated AR42J cell,Ang(1-7)may inhibits p38MAPK/ NF-κB signaling pathway through Mas receptor.