临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2014年
24期
2037-2040
,共4页
胸腔积液%DNA异倍体%肿瘤标志物
胸腔積液%DNA異倍體%腫瘤標誌物
흉강적액%DNA이배체%종류표지물
Pleural effusion%DNA aneuploidy%Tumor markers
目的：探讨DNA异倍体联合肿瘤标志物检测在胸腔积液良恶性诊断中的价值。方法将53例胸腔积液分为恶性组（23例）和良性组（30例）。除常规细胞学检查外，以DNA细胞自动检测分析仪检测患者胸腔积液中的DNA异倍体，采用电化学发光法测定胸腔积液中癌胚抗原（ CEA）、糖类抗原（ CA125）肿瘤标志物含量。比较DNA 异倍体联合肿瘤标志物诊断与细胞学诊断的优劣。结果 CA125（AUC＝0.747）、CEA（AUC＝0.882）、DNA（AUC＝0.776）均有显著的诊断价值（ P ＜0.001）。CA125取值为＞563 U/ml时，最佳诊断灵敏度、特异度分别为72.73％、76.67％；CEA取值为＞2.93μg/ml时，最佳诊断灵敏度、特异度分别为86.96％、86.67％；DNA取值为＞0时，最佳诊断灵敏度、特异度分别为78.26％、73.33％；3个指标具有同样的诊断价值。将DNA异倍体、CEA、CA125检测结果进行联合诊断的灵敏度与单独诊断时的最佳灵敏度（指标CEA，86.96％）相近，同时其特异度优于3个指标独立诊断时特异度。结论DNA异倍体联合CEA、CA125检测诊断恶性胸腔积液灵敏度与单独诊断时的最佳灵敏度相近，但其特异度优于3个指标独立诊断时特异度。具有定量、快速、价廉、易标准化的特点，且操作简单。
目的：探討DNA異倍體聯閤腫瘤標誌物檢測在胸腔積液良噁性診斷中的價值。方法將53例胸腔積液分為噁性組（23例）和良性組（30例）。除常規細胞學檢查外，以DNA細胞自動檢測分析儀檢測患者胸腔積液中的DNA異倍體，採用電化學髮光法測定胸腔積液中癌胚抗原（ CEA）、糖類抗原（ CA125）腫瘤標誌物含量。比較DNA 異倍體聯閤腫瘤標誌物診斷與細胞學診斷的優劣。結果 CA125（AUC＝0.747）、CEA（AUC＝0.882）、DNA（AUC＝0.776）均有顯著的診斷價值（ P ＜0.001）。CA125取值為＞563 U/ml時，最佳診斷靈敏度、特異度分彆為72.73％、76.67％；CEA取值為＞2.93μg/ml時，最佳診斷靈敏度、特異度分彆為86.96％、86.67％；DNA取值為＞0時，最佳診斷靈敏度、特異度分彆為78.26％、73.33％；3箇指標具有同樣的診斷價值。將DNA異倍體、CEA、CA125檢測結果進行聯閤診斷的靈敏度與單獨診斷時的最佳靈敏度（指標CEA，86.96％）相近，同時其特異度優于3箇指標獨立診斷時特異度。結論DNA異倍體聯閤CEA、CA125檢測診斷噁性胸腔積液靈敏度與單獨診斷時的最佳靈敏度相近，但其特異度優于3箇指標獨立診斷時特異度。具有定量、快速、價廉、易標準化的特點，且操作簡單。
목적：탐토DNA이배체연합종류표지물검측재흉강적액량악성진단중적개치。방법장53례흉강적액분위악성조（23례）화량성조（30례）。제상규세포학검사외，이DNA세포자동검측분석의검측환자흉강적액중적DNA이배체，채용전화학발광법측정흉강적액중암배항원（ CEA）、당류항원（ CA125）종류표지물함량。비교DNA 이배체연합종류표지물진단여세포학진단적우렬。결과 CA125（AUC＝0.747）、CEA（AUC＝0.882）、DNA（AUC＝0.776）균유현저적진단개치（ P ＜0.001）。CA125취치위＞563 U/ml시，최가진단령민도、특이도분별위72.73％、76.67％；CEA취치위＞2.93μg/ml시，최가진단령민도、특이도분별위86.96％、86.67％；DNA취치위＞0시，최가진단령민도、특이도분별위78.26％、73.33％；3개지표구유동양적진단개치。장DNA이배체、CEA、CA125검측결과진행연합진단적령민도여단독진단시적최가령민도（지표CEA，86.96％）상근，동시기특이도우우3개지표독립진단시특이도。결론DNA이배체연합CEA、CA125검측진단악성흉강적액령민도여단독진단시적최가령민도상근，단기특이도우우3개지표독립진단시특이도。구유정량、쾌속、개렴、역표준화적특점，차조작간단。
Objective The aim of this study is to explore the diagnostic value of DNA aneuploidy combined with tumor markers in deter-mination of pleural effusions of malignant and benign origin. Methods 53 patients with pleural effusions were divided into the malignant group( n=23)and benign group(n=30). In addition to routine cytology,DNA aneuploidy in pleural effusion was detected with automatic DNA analyzer. The levels of tumor markers CEA and CA125 in pleural effusion were detected by electrochemiluminescence assay. The advantages and disadvanta-ges were compared between diagnostic values of DNA aneuploidy combined with tumor markers and cytology. Results CA125(AUC=0. 747), CEA(AUC=0. 882)and DNA(AUC=0. 776)had significant diagnostic values( P <0. 001). When CA125>563 U/ml,the optimum diag-nostic sensitivity and specificity were 72. 73% and 76. 67%,respectively. When CEA>2. 93 μg/ml,the optimum diagnostic sensitivity and spe-cificity were 86. 96% and 86. 67%,respectively. When DNA>0,the optimum diagnostic sensitivity and specificity were 78. 26% and 73. 33%, respectively. Three indicators had the same diagnostic value. The combined diagnosis of DNA aneuploidy,CEA and CA125 results had a similar sensitivity with the independent diagnosis(optimum sensitivity:CEA=86. 96%)and a superior specificity than the independent diagnosis. Con-clusion The combined diagnosis of DNA aneuploidy,CEA and CA125 results have a similar sensitivity with the independent diagnosis and a su-perior specificity than the independent diagnosis. This method is quantifiable,rapid,inexpensive and standardized,with simple procedures.
