CAJ | 학술논문

目的：探讨中药骨碎补对体外培养的人的牙髓、牙龈、牙周膜成纤维细胞增殖的影响。方法采用组织块法外培养人牙髓、牙龈、牙周膜成纤维细胞，取第4、5代培养细胞随机分为实验组和对照组，实验组加不同浓度的骨碎补，对照组只加培养液。通过HE染色观察其对牙髓、牙龈、牙周膜成纤维细胞的形态、结构及生长增殖的影响，通过MTT法和考马斯亮蓝法对实验组和对照组的细胞生长情况和总蛋白合成的检测。结果①在10~1000μg/ml浓度范围内骨碎补对人牙髓、牙龈、牙周膜成纤维细胞均有促增殖作用（ P ＜0.05），尤以100μg/ml浓度最为明显（ P ＜0.01）；②总蛋白测定结果显示在骨碎补浓度为10μg/ml时，对照组与其总蛋白含量差异无统计学意义（ P ＞0.05），实验组细胞总蛋白含量随骨碎补浓度增加高于对照组（ P ＜0.05），尤以500μg/ml骨碎补浓度组作用牙髓成纤维细胞最佳；而100μg/ml骨碎补浓度组作用牙龈、牙周膜成纤维细胞最显著。结论骨碎补在有效作用浓度范围内，其促增殖效应和总蛋白含量与骨碎补浓度呈剂量依赖关系，随着浓度的增加促增殖作用增强、总蛋白含量增加，浓度达到最大作用后，随着浓度增加作用反而呈现减弱趋势。
목적：탐토중약골쇄보대체외배양적인적아수、아간、아주막성섬유세포증식적영향。방법채용조직괴법외배양인아수、아간、아주막성섬유세포，취제4、5대배양세포수궤분위실험조화대조조，실험조가불동농도적골쇄보，대조조지가배양액。통과HE염색관찰기대아수、아간、아주막성섬유세포적형태、결구급생장증식적영향，통과MTT법화고마사량람법대실험조화대조조적세포생장정황화총단백합성적검측。결과①재10~1000μg/ml농도범위내골쇄보대인아수、아간、아주막성섬유세포균유촉증식작용（ P ＜0.05），우이100μg/ml농도최위명현（ P ＜0.01）；②총단백측정결과현시재골쇄보농도위10μg/ml시，대조조여기총단백함량차이무통계학의의（ P ＞0.05），실험조세포총단백함량수골쇄보농도증가고우대조조（ P ＜0.05），우이500μg/ml골쇄보농도조작용아수성섬유세포최가；이100μg/ml골쇄보농도조작용아간、아주막성섬유세포최현저。결론골쇄보재유효작용농도범위내，기촉증식효응화총단백함량여골쇄보농도정제량의뢰관계，수착농도적증가촉증식작용증강、총단백함량증가，농도체도최대작용후，수착농도증가작용반이정현감약추세。
Objective To observe the effects of Rhizoma drynariae on cell proliferation of human dental pulp,gingival,periodontal liga-ment fibroblasts cells cultured in vitro. Methods Isolated human dental pulp,gingival,periodontal ligament fibroblasts cells were cultured ac-cording to the method of tissue-explant. The fourth,fifth passage cultured cells were selected and randomly divided into experiment group and control group. The prepared Rhizoma Drynariac decoction with different concentration were added in the experiment groups. While,in control groups only culture medium were added. Which applied the Rhizoma Drynariac decoction to the cells in morphology,structure and vitro,the effects were observed by HE staining. The proliferation cells of human dental pulp,gingival,periodontal ligament were evaluated by MTT assay and the synthesis of the total proteins in the cells was measured by the method of Coomassie brilliant blue stain. Results ①The total protein con-tent of human dental pulp,gingival,periodontal ligament fibroblasts cells increased in each experiment group after adding 10~1 000 μg/ml Rhi-zoma drynariae respectively( P <0. 05). The effect of 100μg/ml was excellent( P <0. 01). ②The measurement results are displayed when the total protein concentration in Drynaria 10μg/ml. Its total protein content in the control group had no significant difference( P >0. 05). The total protein content of the experimental group increased with higher concentrations Drynaria( P <0. 05). The concentration of 500 μg/ml was excel-lent on Human dental pulp fibroblasts cells;but the 100 μg/ml was excellent on human gingival fibroblasts cells,periodontal ligament fibroblasts cells. Conclusion Drynaria effective role within the concentration range,the pro-proliferative effects,total protein concentration and dose-de-pendent Drynaria increased with increasing concentrations of total protein content,which can promote proliferation of enhanced concentration and reach a maximum effect,with the concentration increasing role but showing signs of abating.